Detailed information of TA system TA113910

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 1556951..1557507
• Replicon: chromosome
• Accession: NZ_CP033631
• Organism: Klebsiella sp. P1CD1

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: DTA24_RS08005
• Protein ID: WP_123836708.1
• Coordinates: 1557172..1557507 (+)
• Length: 112 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: DTA24_RS08000
• Protein ID: WP_164481324.1
• Coordinates: 1556951..1557181 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DTA24_RS07960	1552483..1553343	+	861	WP_123836700.1	hypothetical protein	-
DTA24_RS07965	1553336..1553938	+	603	WP_123836701.1	hypothetical protein	-
DTA24_RS28605	1553965..1554261	+	297	WP_206060570.1	hypothetical protein	-
DTA24_RS07975	1554258..1554710	+	453	WP_123836702.1	hypothetical protein	-
DTA24_RS07980	1554715..1555263	-	549	WP_123836703.1	hypothetical protein	-
DTA24_RS07985	1555263..1555517	-	255	WP_123836704.1	Arc family DNA-binding protein	-
DTA24_RS07990	1555603..1555764	+	162	WP_123836705.1	Arc family DNA-binding protein	-
DTA24_RS07995	1555833..1556816	+	984	WP_123836706.1	phage antirepressor N-terminal domain-containing protein	-
DTA24_RS08000	1556951..1557181	+	231	WP_164481324.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
DTA24_RS08005	1557172..1557507	+	336	WP_123836708.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
DTA24_RS08010	1557572..1557931	+	360	WP_123836709.1	hypothetical protein	-
DTA24_RS08015	1557939..1559171	+	1233	WP_123836710.1	baseplate J/gp47 family protein	-
DTA24_RS08020	1559171..1559722	+	552	WP_123836711.1	hypothetical protein	-
DTA24_RS08025	1559704..1560591	+	888	WP_123836712.1	hypothetical protein	-
DTA24_RS08030	1560575..1561186	+	612	WP_123836713.1	methyltransferase domain-containing protein	-
DTA24_RS08035	1561189..1561788	+	600	WP_072045291.1	DUF2612 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1501818..1569322	67504

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (6-109)

Antitoxin

• SpoVT-AbrB domain (7-41)

Sequences

Toxin

Protein

• Length: 112 a.a.
• Molecular weight: 12110.93 Da
• Isoelectric Point: 9.9248

>T113910 WP_123836708.1 NZ_CP033631:1557172-1557507 [Klebsiella sp. P1CD1]
ITVTARQGDVVLIDFTPSAGSEQGGKRPALVVSETYFNQHDRFVVLCPITSKAKGYGFEVPIRGTQKTTGVILSNQFKSL
DYRARNMVVVDSVDAKTLAAVQHNIKQILGM

Nucleotide

• Length: 336 bp

>T113910 NZ_CP033631:1557172-1557507 [Klebsiella sp. P1CD1]
ATTACTGTGACAGCCAGGCAAGGAGATGTGGTTTTAATCGACTTCACCCCTTCGGCTGGTAGTGAGCAGGGCGGAAAGCG
TCCTGCTCTTGTGGTTTCAGAGACATATTTCAACCAACACGACAGGTTTGTTGTTTTATGCCCGATCACAAGCAAAGCAA
AAGGCTATGGATTCGAGGTGCCCATAAGGGGCACACAGAAAACGACTGGAGTAATTCTTAGCAATCAGTTTAAGTCATTG
GATTACAGAGCAAGAAACATGGTTGTTGTGGATTCTGTGGATGCCAAAACTCTGGCTGCCGTTCAGCATAACATCAAACA
AATTCTTGGCATGTGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8073.29 Da
• Isoelectric Point: 4.4368

>AT113910 WP_164481324.1 NZ_CP033631:1556951-1557181 [Klebsiella sp. P1CD1]
MATVVAKWGNAVGVRIPAALLDQAGITVGDQVEFCVKGGMLGLKKTGPTLQELMSKCTAENSHDEYFSESMGEELL

Nucleotide

• Length: 231 bp

>AT113910 NZ_CP033631:1556951-1557181 [Klebsiella sp. P1CD1]
ATGGCAACAGTAGTCGCAAAATGGGGCAATGCCGTAGGCGTTCGCATACCTGCGGCGCTACTTGATCAAGCTGGCATCAC
TGTTGGCGATCAGGTTGAGTTCTGTGTTAAGGGTGGGATGCTTGGGCTGAAAAAGACCGGGCCAACACTCCAGGAACTCA
TGTCAAAATGTACAGCAGAAAACAGTCACGATGAGTACTTTTCTGAGTCAATGGGAGAAGAATTACTGTGA