Detailed information of TA system TA113902

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 32173..32816
• Replicon: plasmid unnamed3
• Accession: NZ_CP033628
• Organism: Klebsiella pneumoniae strain 4743

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: D8L2J9
• Locus tag: DPQ49_RS28630
• Protein ID: WP_001044770.1
• Coordinates: 32173..32589 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: D5KTK7
• Locus tag: DPQ49_RS28635
• Protein ID: WP_001261282.1
• Coordinates: 32586..32816 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DPQ49_RS28595	27715..27951	-	237	WP_000993386.1	broad-spectrum mercury transporter MerE	-
DPQ49_RS28600	27948..28310	-	363	WP_001277456.1	mercury resistance co-regulator MerD	-
DPQ49_RS28605	28328..30022	-	1695	WP_000105636.1	mercury(II) reductase	-
DPQ49_RS28610	30074..30496	-	423	WP_001340589.1	organomercurial transporter MerC	-
DPQ49_RS28615	30532..30642	-	111	Protein_31	mercuric transport protein periplasmic component	-
DPQ49_RS28620	30658..31355	-	698	Protein_32	IS1 family transposase	-
DPQ49_RS28625	31389..32099	-	711	WP_004152334.1	ATP-binding protein	-
DPQ49_RS28630	32173..32589	-	417	WP_001044770.1	type II toxin-antitoxin system VapC family toxin	Toxin
DPQ49_RS28635	32586..32816	-	231	WP_001261282.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
DPQ49_RS28640	32773..33234	+	462	WP_072202616.1	hypothetical protein	-
DPQ49_RS28645	33378..33728	+	351	WP_000493378.1	hypothetical protein	-
DPQ49_RS28650	33779..34522	+	744	WP_000129823.1	hypothetical protein	-
DPQ49_RS28655	34519..35295	+	777	WP_000015958.1	site-specific integrase	-
DPQ49_RS28660	35353..35610	-	258	WP_000764642.1	hypothetical protein	-
DPQ49_RS30825	35739..35843	-	105	WP_032409716.1	hypothetical protein	-
DPQ49_RS28665	36378..37244	+	867	WP_004118283.1	replication initiation protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaKPC-2 / blaOXA-9 / blaTEM-1A / mph(E) / msr(E) / armA	-	1..110020	110020

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15106.52 Da
• Isoelectric Point: 7.1084

>T113902 WP_001044770.1 NZ_CP033628:c32589-32173 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Nucleotide

• Length: 417 bp

>T113902 NZ_CP033628:c32589-32173 [Klebsiella pneumoniae]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8756.83 Da
• Isoelectric Point: 4.4692

>AT113902 WP_001261282.1 NZ_CP033628:c32816-32586 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Nucleotide

• Length: 231 bp

>AT113902 NZ_CP033628:c32816-32586 [Klebsiella pneumoniae]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Structures

Toxin

• AlphaFold DB: A0A0H3GYM2

Antitoxin

• AlphaFold DB: A0A0H3GZG3