Detailed information of TA system TA113901

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 52330..53066
• Replicon: plasmid unnamed2
• Accession: NZ_CP033627
• Organism: Klebsiella pneumoniae strain 4743

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: L7SZ15
• Locus tag: DPQ49_RS27685
• Protein ID: WP_003026803.1
• Coordinates: 52584..53066 (+)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: L7SZ29
• Locus tag: DPQ49_RS27680
• Protein ID: WP_003026799.1
• Coordinates: 52330..52596 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DPQ49_RS27635	48392..48754	-	363	WP_004152100.1	arsenite efflux transporter metallochaperone ArsD	-
DPQ49_RS27640	48804..49154	-	351	WP_004152101.1	As(III)-sensing metalloregulatory transcriptional repressor ArsR	-
DPQ49_RS27645	49512..49781	+	270	WP_004152102.1	hypothetical protein	-
DPQ49_RS27650	49769..50344	+	576	WP_004152103.1	hypothetical protein	-
DPQ49_RS27655	50375..50869	+	495	WP_004152104.1	DNA-binding protein	-
DPQ49_RS27660	50913..51281	+	369	WP_004152105.1	hypothetical protein	-
DPQ49_RS27665	51315..51518	+	204	WP_004152106.1	hemolysin expression modulator Hha	-
DPQ49_RS27670	51567..51824	+	258	WP_004152107.1	hypothetical protein	-
DPQ49_RS27675	51900..52154	+	255	WP_004152108.1	hypothetical protein	-
DPQ49_RS27680	52330..52596	+	267	WP_003026799.1	DUF1778 domain-containing protein	Antitoxin
DPQ49_RS27685	52584..53066	+	483	WP_003026803.1	GNAT family N-acetyltransferase	Toxin
DPQ49_RS27690	53267..54670	+	1404	WP_001567368.1	ISNCY-like element ISKpn21 family transposase	-
DPQ49_RS27695	54699..55331	-	633	WP_001567369.1	hypothetical protein	-
DPQ49_RS27700	55561..56907	+	1347	WP_020314316.1	ISNCY family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..184083	184083

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (45-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17353.97 Da
• Isoelectric Point: 9.5822

>T113901 WP_003026803.1 NZ_CP033627:52584-53066 [Klebsiella pneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Nucleotide

• Length: 483 bp

>T113901 NZ_CP033627:52584-53066 [Klebsiella pneumoniae]
GTGGGACGTATAACCACGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGTAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTCCACGATGCAGTTCTACGGTGTTATCGCGTAGCTGAGAACATTGGGGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10147.59 Da
• Isoelectric Point: 4.5256

>AT113901 WP_003026799.1 NZ_CP033627:52330-52596 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT113901 NZ_CP033627:52330-52596 [Klebsiella pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCTGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Structures

Toxin

• AlphaFold DB: A0A0J2GNW6

Antitoxin

• AlphaFold DB: A0A1Q8YL66