Detailed information of TA system TA113899

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 4649806..4650407
• Replicon: chromosome
• Accession: NZ_CP033623
• Organism: Serratia marcescens strain N10A28

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: EFZ62_RS22025
• Protein ID: WP_060424343.1
• Coordinates: 4649806..4650186 (-)
• Length: 127 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A086G6A0
• Locus tag: EFZ62_RS22030
• Protein ID: WP_004933932.1
• Coordinates: 4650186..4650407 (-)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EFZ62_RS22000	4645059..4646339	+	1281	WP_159318801.1	DUF3748 domain-containing protein	-
EFZ62_RS22005	4646370..4646717	-	348	WP_016929593.1	YceK/YidQ family lipoprotein	-
EFZ62_RS22010	4647027..4647440	+	414	WP_004933919.1	heat shock chaperone IbpA	-
EFZ62_RS22015	4647541..4647969	+	429	WP_004933922.1	heat shock chaperone IbpB	-
EFZ62_RS22020	4648137..4649795	+	1659	WP_084827017.1	putative transporter	-
EFZ62_RS22025	4649806..4650186	-	381	WP_060424343.1	type II toxin-antitoxin system VapC family toxin	Toxin
EFZ62_RS22030	4650186..4650407	-	222	WP_004933932.1	ribbon-helix-helix domain-containing protein	Antitoxin
EFZ62_RS22035	4650483..4651733	-	1251	WP_004933935.1	valine--pyruvate transaminase	-
EFZ62_RS22040	4651869..4653932	-	2064	WP_159318802.1	alpha-amylase	-
EFZ62_RS25075	4654128..4654280	+	153	WP_169317907.1	hypothetical protein	-
EFZ62_RS22045	4654282..4655259	-	978	WP_060424337.1	glyoxylate/hydroxypyruvate reductase GhrB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-102)

Antitoxin

• Ribbon-helix-helix protein, CopG (3-40)

Sequences

Toxin

Protein

• Length: 127 a.a.
• Molecular weight: 14213.58 Da
• Isoelectric Point: 7.3170

>T113899 WP_060424343.1 NZ_CP033623:c4650186-4649806 [Serratia marcescens]
MVVQRALFDTNILIDYLNGIPQAKDVLTEYHINPAISAITWMEVMVGAKKQGPALELKTRQFLGQFLLLPITDEVAERAV
ELRHSQHVKLPDAIIWATAQVGFRTLISRNPKDFGTDNGVLMPYRL

Nucleotide

• Length: 381 bp

>T113899 NZ_CP033623:c4650186-4649806 [Serratia marcescens]
ATGGTGGTTCAACGGGCGCTATTCGACACCAATATCCTGATCGATTATCTCAACGGCATTCCTCAAGCCAAAGACGTTCT
GACCGAATACCACATCAACCCGGCTATTAGCGCCATCACCTGGATGGAAGTGATGGTGGGTGCCAAAAAACAGGGGCCGG
CGCTGGAGCTGAAAACGCGCCAGTTTTTAGGGCAATTCCTGCTGCTGCCTATTACCGATGAAGTTGCCGAACGCGCCGTA
GAACTACGCCACTCACAGCATGTGAAGCTGCCGGACGCCATTATCTGGGCCACCGCGCAGGTGGGGTTCCGGACGCTCAT
CTCCCGCAACCCCAAAGATTTTGGCACTGACAACGGCGTGTTAATGCCTTACCGGCTGTAG

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8593.53 Da
• Isoelectric Point: 4.4791

>AT113899 WP_004933932.1 NZ_CP033623:c4650407-4650186 [Serratia marcescens]
MTRIVIDLPEEDLRQLDNLKNIRHVPRAEIIRQAVSRYLTENHVEDPSNAFGLWSGAMGDGVDYENRQREEWE

Nucleotide

• Length: 222 bp

>AT113899 NZ_CP033623:c4650407-4650186 [Serratia marcescens]
ATGACCCGCATCGTGATCGATTTACCTGAAGAAGACCTGCGGCAGCTCGACAATTTGAAAAATATCCGCCACGTTCCCCG
GGCCGAAATCATCCGTCAGGCCGTCTCCCGTTATCTGACGGAGAACCACGTTGAAGATCCCAGCAATGCTTTCGGGCTCT
GGTCCGGGGCGATGGGCGATGGCGTAGACTACGAAAATCGTCAGCGCGAGGAGTGGGAATAA

Structures

Antitoxin

• AlphaFold DB: A0A086G6A0