Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 422045..422667 Replicon chromosome
Accession NZ_CP033623
Organism Serratia marcescens strain N10A28

Toxin (Protein)


Gene name Hha Uniprot ID A0A8B4G7F9
Locus tag EFZ62_RS02020 Protein ID WP_004940313.1
Coordinates 422045..422248 (-) Length 68 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID A0A2X2G5J9
Locus tag EFZ62_RS02025 Protein ID WP_004940312.1
Coordinates 422299..422667 (-) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EFZ62_RS01995 417855..418301 + 447 WP_159317709.1 type II secretion system protein M -
EFZ62_RS02000 418337..419152 + 816 WP_159317710.1 A24 family peptidase -
EFZ62_RS02005 419149..419511 - 363 WP_159317711.1 type II secretion system pilot lipoprotein GspS -
EFZ62_RS02010 419764..421191 + 1428 WP_159317712.1 N-acetylglucosamine-binding protein GbpA -
EFZ62_RS02015 421244..421762 + 519 WP_060423424.1 cytochrome b/b6 domain-containing protein -
EFZ62_RS02020 422045..422248 - 204 WP_004940313.1 hemolysin expression modulator Hha Toxin
EFZ62_RS02025 422299..422667 - 369 WP_004940312.1 Hha toxicity modulator TomB Antitoxin
EFZ62_RS02030 422826..423179 - 354 WP_060423427.1 hypothetical protein -
EFZ62_RS02035 423452..423592 + 141 WP_154292170.1 hypothetical protein -
EFZ62_RS02040 423589..424302 + 714 WP_060423430.1 ABC transporter ATP-binding protein -
EFZ62_RS02045 424299..425156 + 858 WP_060440564.1 metal ABC transporter permease -
EFZ62_RS02050 425182..426060 + 879 WP_089185570.1 metal ABC transporter substrate-binding protein -
EFZ62_RS02055 426167..426307 - 141 WP_004940304.1 type B 50S ribosomal protein L36 -
EFZ62_RS02060 426320..426574 - 255 WP_004940303.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(8-64)

Antitoxin

Biofilm formation regulator YbaJ

(1-118)


Sequences

Toxin        


Download         Length: 68 a.a.        Molecular weight: 8107.37 Da        Isoelectric Point: 6.9756

>T113882 WP_004940313.1 NZ_CP033623:c422248-422045 [Serratia marcescens]
MTKTDYLMRLRKCTTIDTLERVIEKNKYELSDDELELFYSAADHRLAELTMNKLYDKIPTSVWKYVR

Download         Length: 204 bp

>T113882 NZ_CP033623:c422248-422045 [Serratia marcescens]
ATGACTAAAACCGACTATCTGATGCGTTTACGTAAATGCACCACGATTGATACTCTCGAACGTGTTATTGAGAAAAATAA
GTACGAGCTTTCCGATGATGAACTGGAGCTGTTTTACTCAGCGGCCGATCACCGTCTGGCGGAGCTCACCATGAACAAAC
TGTATGACAAAATCCCCACCTCCGTTTGGAAATATGTCAGGTAA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 14261.95 Da        Isoelectric Point: 4.4314

>AT113882 WP_004940312.1 NZ_CP033623:c422667-422299 [Serratia marcescens]
MDEYSPKRHDIAQLKFLCENLYDEGIATLGDSHHGWVNDPTSSVNLQLNELIEHIASFVMSYKIKYMDESDLSELVEEYL
DDTYTLFSSYGINDSDLRRWQKTKARLFRMFSGEDICTTMKT

Download         Length: 369 bp

>AT113882 NZ_CP033623:c422667-422299 [Serratia marcescens]
ATGGATGAGTATTCGCCTAAGCGACACGACATTGCTCAGCTCAAATTCTTGTGCGAAAATTTGTATGATGAAGGCATCGC
GACCCTGGGAGACAGCCACCACGGGTGGGTCAACGATCCGACATCCTCCGTCAACCTGCAGCTTAATGAGTTGATCGAAC
ATATCGCTTCGTTTGTGATGAGTTATAAAATTAAATACATGGACGAATCAGACCTGTCGGAGTTAGTCGAAGAATATCTC
GACGATACTTATACGCTGTTCAGCAGCTACGGTATCAATGATTCCGACCTGCGCCGCTGGCAAAAGACCAAGGCGAGATT
ATTCAGAATGTTCTCAGGAGAGGACATCTGTACGACAATGAAAACTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A8B4G7F9

Antitoxin

Source ID Structure
AlphaFold DB A0A2X2G5J9

References