Detailed information of TA system TA113876

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): DhiAT/dnstrm_HI1420(antitoxin)
• Location: 3041094..3041844
• Replicon: chromosome
• Accession: NZ_CP033622
• Organism: Dickeya zeae strain CE1

Toxin (Protein)

• Gene name: dhiT
• Uniprot ID: -
• Locus tag: DWG24_RS13585
• Protein ID: WP_168362935.1
• Coordinates: 3041587..3041844 (-)
• Length: 86 a.a.

Antitoxin (Protein)

• Gene name: dhiA
• Uniprot ID: -
• Locus tag: DWG24_RS13580
• Protein ID: WP_168362934.1
• Coordinates: 3041094..3041579 (-)
• Length: 162 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DWG24_RS13555	3037032..3038759	+	1728	WP_168362930.1	hydrogenase large subunit	-
DWG24_RS13560	3038770..3039339	+	570	WP_016943631.1	hydrogenase 4 subunit H	-
DWG24_RS13565	3039336..3040124	+	789	WP_168362931.1	NADH-quinone oxidoreductase subunit B family protein	-
DWG24_RS13570	3040124..3040522	+	399	WP_168362932.1	formate hydrogenlyase maturation HycH family protein	-
DWG24_RS13575	3040515..3041009	+	495	WP_168362933.1	hydrogenase maturation peptidase HycI	-
DWG24_RS13580	3041094..3041579	-	486	WP_168362934.1	addiction module antitoxin	Antitoxin
DWG24_RS13585	3041587..3041844	-	258	WP_168362935.1	DUF4160 domain-containing protein	Toxin
DWG24_RS13590	3042394..3042936	+	543	WP_016943624.1	4Fe-4S dicluster domain-containing protein	-
DWG24_RS13595	3042971..3045121	+	2151	WP_168362936.1	formate dehydrogenase subunit alpha	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Protein of unknown function DUF4160 (9-73)

Antitoxin

• Uncharacterized protein HI1420 (80-156)

Sequences

Toxin

Protein

• Length: 86 a.a.
• Molecular weight: 9865.48 Da
• Isoelectric Point: 7.5161

>T113876 WP_168362935.1 NZ_CP033622:c3041844-3041587 [Dickeya zeae]
MPEIDRLAGLIFYLYFYDDERHHLPHVHVCYGNHELIISIRTGTEIEGYLPNTKRKLAIAHIMAHREQLLSMWAQAVIGK
NPGKL

Nucleotide

• Length: 258 bp

>T113876 NZ_CP033622:c3041844-3041587 [Dickeya zeae]
ATGCCTGAGATTGACAGACTGGCAGGGTTAATCTTCTATTTGTACTTTTACGATGATGAGCGGCACCACTTGCCGCATGT
GCATGTCTGCTACGGCAATCATGAACTTATCATCAGTATTCGTACCGGAACCGAGATAGAGGGATATCTGCCAAATACCA
AACGTAAGCTGGCGATTGCCCATATCATGGCGCATCGTGAGCAATTGCTGAGTATGTGGGCACAGGCGGTTATCGGGAAG
AATCCGGGAAAATTGTGA

Antitoxin

Protein

• Length: 162 a.a.
• Molecular weight: 17779.04 Da
• Isoelectric Point: 6.8503

>AT113876 WP_168362934.1 NZ_CP033622:c3041579-3041094 [Dickeya zeae]
MHKIVDVDVTGDFSLRLTYDDGLISDVNLYHYASHSVFSHASQFIRFGLLPDGSLEWPDITLSSSALREKGTYVGHTLAV
RERNFADIISRAFYDAVIENRPEILQAALKTAVDKHGNSTVAKEAGAKSRTSLYKSLNKNTNVSLATVVSLAHTVLQLEE
K

Nucleotide

• Length: 486 bp

>AT113876 NZ_CP033622:c3041579-3041094 [Dickeya zeae]
ATGCATAAGATTGTAGATGTTGACGTGACTGGTGACTTTTCGTTGCGTCTGACTTATGACGATGGCCTGATAAGCGACGT
TAACCTGTATCACTATGCTTCACATTCTGTGTTTTCGCATGCCAGCCAGTTTATTCGATTTGGGTTATTACCTGATGGTT
CGCTGGAATGGCCGGATATCACGCTCTCTTCGTCCGCATTACGGGAAAAGGGGACGTATGTTGGCCACACATTGGCGGTG
AGGGAGCGAAATTTTGCCGATATTATCTCCCGAGCTTTCTATGATGCCGTGATTGAGAACCGGCCAGAAATTCTGCAGGC
GGCGCTGAAAACCGCAGTAGACAAACATGGCAACAGTACGGTGGCCAAAGAAGCGGGAGCCAAAAGCCGTACCAGCTTGT
ATAAATCGCTGAATAAAAACACTAATGTCAGCCTGGCGACGGTAGTTAGCCTGGCCCATACCGTGTTGCAGTTGGAAGAG
AAATAG