Detailed information of TA system TA113875

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 2895345..2895898
• Replicon: chromosome
• Accession: NZ_CP033622
• Organism: Dickeya zeae strain CE1

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: -
• Locus tag: DWG24_RS12890
• Protein ID: WP_168362834.1
• Coordinates: 2895345..2895659 (-)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: D2BWS8
• Locus tag: DWG24_RS12895
• Protein ID: WP_012884146.1
• Coordinates: 2895662..2895898 (-)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DWG24_RS12860	2890595..2891629	+	1035	WP_168362830.1	amidohydrolase family protein	-
DWG24_RS12865	2891782..2892339	+	558	WP_168362831.1	NAD(P)H-dependent oxidoreductase	-
DWG24_RS12870	2892358..2893377	+	1020	WP_168362832.1	aldo/keto reductase	-
DWG24_RS12875	2893437..2894132	+	696	WP_168364072.1	antibiotic biosynthesis monooxygenase	-
DWG24_RS12880	2894148..2894951	+	804	WP_168362833.1	carboxymuconolactone decarboxylase family protein	-
DWG24_RS12885	2895094..2895348	+	255	WP_168364073.1	mobilization protein mobC	-
DWG24_RS12890	2895345..2895659	-	315	WP_168362834.1	CcdB family protein	Toxin
DWG24_RS12895	2895662..2895898	-	237	WP_012884146.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
DWG24_RS12900	2896219..2896926	-	708	Protein_2507	DUF1738 domain-containing protein	-
DWG24_RS12905	2896947..2897600	+	654	WP_205905869.1	hypothetical protein	-
DWG24_RS12910	2897629..2897871	+	243	WP_205905870.1	hypothetical protein	-
DWG24_RS12920	2898775..2900001	+	1227	Protein_2511	amidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-103)

Antitoxin

• Post-segregation antitoxin CcdA (9-78)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11488.38 Da
• Isoelectric Point: 7.9859

>T113875 WP_168362834.1 NZ_CP033622:c2895659-2895345 [Dickeya zeae]
MQFTVYGNTGKSAIYPLLLDVTSDIIGQLNRRIVIPLLPVEKYPAGRRPDRLVPVVRLTDGKEYAVMTHELASSPVQALG
AVFCDASQYRNQVKVAIDFLIDGI

Nucleotide

• Length: 315 bp

>T113875 NZ_CP033622:c2895659-2895345 [Dickeya zeae]
ATGCAATTCACCGTATACGGTAATACCGGGAAAAGCGCCATTTACCCGCTGTTGCTCGATGTCACGAGCGATATTATTGG
GCAGTTGAATCGTCGGATAGTGATCCCGTTGCTCCCTGTTGAAAAGTACCCGGCAGGTCGCCGACCGGATCGCCTTGTCC
CTGTCGTCAGGCTGACGGACGGCAAAGAATACGCCGTAATGACCCACGAGCTGGCCAGTAGCCCTGTGCAGGCCCTGGGG
GCTGTGTTTTGTGATGCGTCGCAGTACCGTAATCAGGTAAAGGTCGCAATAGATTTCCTCATCGACGGCATTTAG

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 9062.10 Da
• Isoelectric Point: 6.4629

>AT113875 WP_012884146.1 NZ_CP033622:c2895898-2895662 [Dickeya zeae]
MTAKQRNTQSVTMTVERALLVRAREAGINLSATLSTALDAELRRYEAKKWQEENREAIDALNRFHDEHGCFSDEYRTF

Nucleotide

• Length: 237 bp

>AT113875 NZ_CP033622:c2895898-2895662 [Dickeya zeae]
ATGACCGCGAAACAACGTAACACGCAGAGCGTGACCATGACGGTAGAGCGTGCCTTACTGGTCCGGGCGCGTGAAGCGGG
CATTAACCTGAGTGCCACGCTGTCAACCGCCCTGGATGCGGAGCTTCGCCGTTATGAGGCGAAAAAATGGCAGGAAGAGA
ACAGGGAGGCCATCGACGCATTAAACCGTTTTCATGATGAGCACGGCTGTTTCAGCGATGAATACCGGACGTTTTAA

Structures

Antitoxin

• AlphaFold DB: D2BWS8