Detailed information of TA system TA113861

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PemK-AbrB
• Location: 1561381..1561930
• Replicon: chromosome
• Accession: NZ_CP033611
• Organism: Rickettsiales endosymbiont of Stachyamoeba lipophora strain RICK01

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: EF513_RS07155
• Protein ID: WP_164503861.1
• Coordinates: 1561595..1561930 (+)
• Length: 112 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: EF513_RS07150
• Protein ID: WP_125216711.1
• Coordinates: 1561381..1561605 (+)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EF513_RS07140	1557919..1558983	-	1065	WP_125216709.1	hypothetical protein	-
EF513_RS07145	1559078..1560916	+	1839	WP_125216710.1	translational GTPase TypA	-
EF513_RS07150	1561381..1561605	+	225	WP_125216711.1	type II toxin-antitoxin system PrlF family antitoxin	Antitoxin
EF513_RS07155	1561595..1561930	+	336	WP_164503861.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
EF513_RS07160	1562020..1564776	-	2757	WP_125216713.1	hypothetical protein	-
EF513_RS07165	1564928..1566328	-	1401	WP_164503862.1	phage terminase large subunit	-
EF513_RS07170	1566309..1566662	-	354	WP_125216715.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (5-108)

Antitoxin

• PrlF antitoxin (4-45)

Sequences

Toxin

Protein

• Length: 112 a.a.
• Molecular weight: 12740.88 Da
• Isoelectric Point: 10.5893

>T113861 WP_164503861.1 NZ_CP033611:1561595-1561930 [Rickettsiales endosymbiont of Stachyamoeba lipophora]
VIYNQFDIVKIPFPFNGNDNTKNRPAIVISSTTYQINNNNAILAMITSLNQSKWLEDIPLTNLERTGLSSPSKIRLKLFT
LDQRFIINKIGELAALDRFKLQKHLKQIMLP

Nucleotide

• Length: 336 bp

>T113861 NZ_CP033611:1561595-1561930 [Rickettsiales endosymbiont of Stachyamoeba lipophora]
GTGATTTATAACCAATTTGATATTGTAAAAATTCCTTTTCCTTTTAATGGTAACGATAATACTAAAAACCGTCCTGCGAT
TGTTATTTCTAGCACTACCTACCAAATTAATAATAATAACGCTATTTTAGCTATGATTACTAGCCTGAATCAAAGTAAAT
GGCTTGAGGATATACCGCTAACTAATTTAGAACGCACTGGTCTTTCTTCTCCTTCTAAAATAAGGCTTAAGCTCTTTACC
TTGGACCAAAGATTTATTATTAATAAAATTGGGGAATTAGCCGCCTTGGATAGATTTAAGCTACAAAAACATTTAAAGCA
AATTATGTTACCTTAG

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8402.44 Da
• Isoelectric Point: 4.6881

>AT113861 WP_125216711.1 NZ_CP033611:1561381-1561605 [Rickettsiales endosymbiont of Stachyamoeba lipophora]
MNTSNVTQKFQATVPLSIRKHLGINSGDKVAYEIIDGKVILSKFRPLDVDFIKSVETTLSEWQSDNDDEAYRDL

Nucleotide

• Length: 225 bp

>AT113861 NZ_CP033611:1561381-1561605 [Rickettsiales endosymbiont of Stachyamoeba lipophora]
ATGAATACTTCAAACGTAACACAAAAATTTCAAGCGACCGTACCACTAAGCATTAGAAAACATCTGGGTATAAATAGTGG
CGATAAAGTCGCTTATGAGATTATTGATGGAAAAGTTATTTTAAGTAAGTTTAGACCGCTTGATGTAGACTTTATCAAAT
CGGTTGAAACCACCTTAAGCGAATGGCAATCAGATAATGATGATGAGGCCTATCGTGATTTATAA