Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazE-higA/MazF-HigA
Location 1299621..1300109 Replicon chromosome
Accession NZ_CP033611
Organism Rickettsiales endosymbiont of Stachyamoeba lipophora strain RICK01

Toxin (Protein)


Gene name mazE Uniprot ID -
Locus tag EF513_RS05960 Protein ID WP_206425192.1
Coordinates 1299621..1299947 (+) Length 109 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag EF513_RS05965 Protein ID WP_125216861.1
Coordinates 1299948..1300109 (+) Length 54 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EF513_RS05945 1295984..1298488 - 2505 WP_125216489.1 hypothetical protein -
EF513_RS07935 1298659..1298811 - 153 WP_164503848.1 hypothetical protein -
EF513_RS05950 1299175..1299315 + 141 WP_125216490.1 DUF4113 domain-containing protein -
EF513_RS05955 1299385..1299627 + 243 WP_125216491.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
EF513_RS05960 1299621..1299947 + 327 WP_206425192.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
EF513_RS05965 1299948..1300109 + 162 WP_125216861.1 HigA family addiction module antidote protein Antitoxin
EF513_RS05970 1300098..1300889 - 792 WP_125216493.1 ankyrin repeat domain-containing protein -
EF513_RS05975 1301000..1301158 + 159 WP_125216862.1 HigA family addiction module antidote protein -
EF513_RS05980 1301395..1302387 - 993 WP_125216494.1 ankyrin repeat domain-containing protein -
EF513_RS05985 1302793..1303311 + 519 WP_125216495.1 hypothetical protein -
EF513_RS05990 1303500..1304051 + 552 WP_125216496.1 hypothetical protein -
EF513_RS05995 1304600..1304902 - 303 WP_125216497.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(6-107)

Antitoxin

Cro/C1-type helix-turn-helix domain

(3-32)


Sequences

Toxin        


Download         Length: 109 a.a.        Molecular weight: 12228.58 Da        Isoelectric Point: 10.0432

>T113860 WP_206425192.1 NZ_CP033611:1299621-1299947 [Rickettsiales endosymbiont of Stachyamoeba lipophora]
MVTAYKQFAIFLANLDPTLGSEIKKTRPCVIISPDEMNKYLNTVIIAPLTSVARMYPTRVSCIFDNKKGQIALDQMRTVD
KTRLVKIIGEVKNLESSMIKKTLIEMFS

Download         Length: 327 bp

>T113860 NZ_CP033611:1299621-1299947 [Rickettsiales endosymbiont of Stachyamoeba lipophora]
ATGGTAACAGCATACAAGCAGTTTGCTATTTTTTTAGCAAACCTAGATCCTACATTAGGTAGTGAGATTAAAAAAACCAG
ACCTTGTGTTATTATTTCTCCAGATGAGATGAATAAATACTTAAATACAGTTATTATTGCTCCTTTAACATCTGTTGCTC
GTATGTATCCTACGCGGGTAAGTTGTATCTTCGATAATAAGAAAGGACAAATAGCTTTAGATCAGATGAGAACAGTAGAT
AAAACTAGGCTAGTAAAAATTATTGGTGAAGTCAAAAATCTTGAATCATCAATGATAAAAAAAACTTTAATAGAAATGTT
TTCGTAA

Antitoxin


Download         Length: 54 a.a.        Molecular weight: 6063.10 Da        Isoelectric Point: 11.1135

>AT113860 WP_125216861.1 NZ_CP033611:1299948-1300109 [Rickettsiales endosymbiont of Stachyamoeba lipophora]
IGRINQIINGKRVITAETALILARYFGTTPQFWLNLQNNYDFKVAAANLKLIR

Download         Length: 162 bp

>AT113860 NZ_CP033611:1299948-1300109 [Rickettsiales endosymbiont of Stachyamoeba lipophora]
ATAGGAAGAATTAATCAGATTATAAATGGTAAAAGAGTTATTACTGCAGAGACAGCTTTGATTCTCGCAAGATATTTTGG
TACTACACCACAATTCTGGTTAAATTTACAGAATAATTATGATTTCAAAGTAGCTGCTGCTAATTTAAAACTAATTAGGT
GA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References