Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) chpSB/PRK09812-ChpS
Location 5376833..5377428 Replicon chromosome
Accession NZ_CP033605
Organism Escherichia coli O157:H7 strain TR01

Toxin (Protein)


Gene name chpB Uniprot ID A0A827ISR3
Locus tag EEL45_RS27795 Protein ID WP_061359519.1
Coordinates 5377078..5377428 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name chpS Uniprot ID A0A240D6C8
Locus tag EEL45_RS27790 Protein ID WP_001223212.1
Coordinates 5376833..5377084 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EEL45_RS27780 5372498..5376277 + 3780 WP_123178823.1 autotransporter assembly complex protein TamB -
EEL45_RS27785 5376280..5376621 + 342 WP_001219160.1 gamma-glutamylcyclotransferase -
EEL45_RS27790 5376833..5377084 + 252 WP_001223212.1 type II toxin-antitoxin system antitoxin ChpS Antitoxin
EEL45_RS27795 5377078..5377428 + 351 WP_061359519.1 endoribonuclease toxin ChpB Toxin
EEL45_RS27800 5377505..5378035 - 531 WP_000055070.1 inorganic diphosphatase -
EEL45_RS27805 5378345..5379301 + 957 WP_123178824.1 galactofuranose ABC transporter substrate-binding protein YtfQ -
EEL45_RS27810 5379441..5380943 + 1503 WP_113828126.1 sugar ABC transporter ATP-binding protein -
EEL45_RS27815 5380957..5381979 + 1023 WP_001387274.1 ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(6-114)

Antitoxin

Antitoxin PemI-like

(1-83)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12506.43 Da        Isoelectric Point: 6.4786

>T113859 WP_061359519.1 NZ_CP033605:5377078-5377428 [Escherichia coli O157:H7]
MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLGMTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLV
NQVRMMDLHARQAKRIGLAADEVVEEALLRLQAVVK

Download         Length: 351 bp

>T113859 NZ_CP033605:5377078-5377428 [Escherichia coli O157:H7]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGCGGATGATGGATCTACACGCCCGGCAGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGC
GTTATTACGCTTGCAGGCGGTGGTGAAATAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9301.61 Da        Isoelectric Point: 4.6509

>AT113859 WP_001223212.1 NZ_CP033605:5376833-5377084 [Escherichia coli O157:H7]
MRITIKRWGNSSGMVIPNVVMKELNLRPGQSVEAQVSNNQLILTPISRRYSLDELLAQCDMNAAELSEQDVWGKSTPAGD
EIW

Download         Length: 252 bp

>AT113859 NZ_CP033605:5376833-5377084 [Escherichia coli O157:H7]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTTCAGGTATGGTCATTCCTAATGTTGTGATGAAAGAACTTAACTTACG
GCCGGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCTACCCCTGCGGGTGAC
GAAATATGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A827ISR3

Antitoxin

Source ID Structure
AlphaFold DB A0A240D6C8

References