Detailed information of TA system TA113842

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): paaR-paaA-parE/-
• Location: 2153210..2153681
• Replicon: chromosome
• Accession: NZ_CP033605
• Organism: Escherichia coli O157:H7 strain TR01

Toxin (Protein)

• Gene name: parE_1
• Uniprot ID: A0A0D7C2L1
• Locus tag: EEL45_RS11150
• Protein ID: WP_001303511.1
• Coordinates: 2153403..2153681 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: paaA
• Uniprot ID: Q8XAD5
• Locus tag: EEL45_RS11145
• Protein ID: WP_001302048.1
• Coordinates: 2153210..2153401 (+)
• Length: 64 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EEL45_RS11105	2148414..2148998	-	585	WP_000206793.1	DUF551 domain-containing protein	-
EEL45_RS11110	2149055..2149450	-	396	WP_001118159.1	DUF977 family protein	-
EEL45_RS11115	2149466..2150235	-	770	Protein_2107	DUF1627 domain-containing protein	-
EEL45_RS11120	2150261..2151001	-	741	WP_000788938.1	ATP-binding protein	-
EEL45_RS11125	2151008..2151973	-	966	WP_123178860.1	helix-turn-helix domain-containing protein	-
EEL45_RS11130	2151996..2152421	-	426	WP_000693943.1	Rha family transcriptional regulator	-
EEL45_RS11135	2152418..2152720	-	303	WP_000172738.1	transcriptional regulator	-
EEL45_RS11140	2152818..2153189	+	372	WP_001169686.1	hypothetical protein	-
EEL45_RS11145	2153210..2153401	+	192	WP_001302048.1	hypothetical protein	Antitoxin
EEL45_RS11150	2153403..2153681	+	279	WP_001303511.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
EEL45_RS11155	2154034..2154333	+	300	WP_001240334.1	hypothetical protein	-
EEL45_RS11160	2154405..2154623	+	219	WP_001171930.1	hypothetical protein	-
EEL45_RS11165	2154627..2154791	-	165	WP_001331716.1	hypothetical protein	-
EEL45_RS11170	2155192..2155380	+	189	WP_000449192.1	cell division inhibition protein DicB	-
EEL45_RS11175	2155377..2155568	+	192	WP_001090200.1	DUF1482 family protein	-
EEL45_RS11180	2155661..2156746	+	1086	Protein_2120	exonuclease	-
EEL45_RS11185	2156797..2158010	+	1214	WP_162829202.1	IS3 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	nleG7'	2068159..2159959	91800

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-91)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10661.25 Da
• Isoelectric Point: 5.5647

>T113842 WP_001303511.1 NZ_CP033605:2153403-2153681 [Escherichia coli O157:H7]
MLPVLWLESADTDLDDITSYIARFDIDAAERLWQRLRGCVLPLSEHPYLYPPSDRVPGLREIVAHPNYIILYRVTTSSVE
VVNVIHARRQFP

Nucleotide

• Length: 279 bp

>T113842 NZ_CP033605:2153403-2153681 [Escherichia coli O157:H7]
ATGTTACCCGTGTTATGGCTTGAAAGCGCAGATACCGACCTAGATGATATAACTAGTTATATTGCTCGTTTCGACATAGA
TGCGGCTGAACGCTTATGGCAGCGATTAAGGGGTTGTGTGCTGCCGTTATCCGAACATCCGTATTTATACCCACCAAGCG
ACAGAGTACCTGGCTTGCGTGAGATTGTAGCCCACCCTAACTATATAATTCTATACCGCGTAACAACATCAAGCGTTGAA
GTAGTAAACGTGATCCACGCAAGACGCCAGTTTCCCTAA

Antitoxin

Protein

• Length: 64 a.a.
• Molecular weight: 7463.47 Da
• Isoelectric Point: 8.6828

>AT113842 WP_001302048.1 NZ_CP033605:2153210-2153401 [Escherichia coli O157:H7]
MNRALSPMVSEFETIEQENSYNEWLRAKVATSLADPRPAIPHDEVERRMAERFAKMRKERSKQ

Nucleotide

• Length: 192 bp

>AT113842 NZ_CP033605:2153210-2153401 [Escherichia coli O157:H7]
ATGAATAGAGCCCTTTCACCAATGGTTTCTGAATTTGAAACCATTGAACAAGAAAACAGTTACAACGAATGGCTGCGTGC
GAAAGTAGCAACGAGCCTTGCAGATCCGCGCCCAGCAATTCCCCATGACGAAGTTGAGCGCAGAATGGCAGAACGCTTTG
CTAAGATGCGCAAGGAACGGAGCAAGCAGTAA

Structures

Toxin

• PDB: 5CZF
• PDB: 5CW7
• PDB: 5CZE

Antitoxin

• PDB: 5CZF
• PDB: 3ZBE
• PDB: 5CW7
• AlphaFold DB: A0A829CTP0