Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 1940057..1940695 Replicon chromosome
Accession NZ_CP033605
Organism Escherichia coli O157:H7 strain TR01

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag EEL45_RS09955 Protein ID WP_000813794.1
Coordinates 1940057..1940233 (+) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag EEL45_RS09960 Protein ID WP_001270286.1
Coordinates 1940279..1940695 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EEL45_RS09935 1935679..1936890 - 1212 WP_123178315.1 BenE family transporter YdcO -
EEL45_RS09940 1936943..1937479 + 537 WP_000429147.1 DNA-binding transcriptional regulator SutR -
EEL45_RS09945 1937552..1939513 + 1962 WP_123178316.1 23S rRNA 5-hydroxycytidine C2501 synthase -
EEL45_RS09950 1939605..1939835 - 231 WP_024192036.1 YncJ family protein -
EEL45_RS09955 1940057..1940233 + 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
EEL45_RS09960 1940279..1940695 + 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
EEL45_RS09965 1940774..1942180 + 1407 WP_123178317.1 PLP-dependent aminotransferase family protein -
EEL45_RS09970 1942426..1943571 + 1146 WP_047644524.1 ABC transporter substrate-binding protein -
EEL45_RS09975 1943589..1944602 + 1014 WP_123178318.1 ABC transporter ATP-binding protein -
EEL45_RS09980 1944603..1945544 + 942 WP_123178319.1 ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T113841 WP_000813794.1 NZ_CP033605:1940057-1940233 [Escherichia coli O157:H7]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T113841 NZ_CP033605:1940057-1940233 [Escherichia coli O157:H7]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT113841 WP_001270286.1 NZ_CP033605:1940279-1940695 [Escherichia coli O157:H7]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT113841 NZ_CP033605:1940279-1940695 [Escherichia coli O157:H7]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCTAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References