Detailed information of TA system TA113830

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/upstrm_HI1419-dnstrm_HI1420
• Location: 49459..50060
• Replicon: chromosome
• Accession: NZ_CP033600
• Organism: Pasteurella multocida strain CQ6

Toxin (Protein)

• Gene name: relE
• Uniprot ID: V4NB29
• Locus tag: PmCQ6_RS00310
• Protein ID: WP_016570073.1
• Coordinates: 49459..49773 (+)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: PmCQ6_RS00315
• Protein ID: WP_016570074.1
• Coordinates: 49770..50060 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PmCQ6_RS00285	44698..45066	-	369	Protein_52	Bro-N domain-containing protein	-
PmCQ6_RS00290	45528..46061	-	534	WP_014391102.1	hypothetical protein	-
PmCQ6_RS00295	46149..46412	-	264	WP_102827045.1	hypothetical protein	-
PmCQ6_RS00300	46509..47759	-	1251	WP_081375317.1	SIR2 family protein	-
PmCQ6_RS00305	48447..48737	-	291	WP_102827047.1	hypothetical protein	-
PmCQ6_RS11155	49044..49196	-	153	WP_197417893.1	hypothetical protein	-
PmCQ6_RS00310	49459..49773	+	315	WP_016570073.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
PmCQ6_RS00315	49770..50060	+	291	WP_016570074.1	putative addiction module antidote protein	Antitoxin
PmCQ6_RS00320	50085..50549	-	465	WP_080673118.1	hypothetical protein	-
PmCQ6_RS00325	50560..50955	-	396	WP_016570076.1	hypothetical protein	-
PmCQ6_RS00330	51023..51679	-	657	WP_016570077.1	XRE family transcriptional regulator	-
PmCQ6_RS00335	51810..52016	+	207	WP_016570078.1	helix-turn-helix transcriptional regulator	-
PmCQ6_RS00340	52065..52517	+	453	WP_016570079.1	hypothetical protein	-
PmCQ6_RS00345	52575..52835	+	261	WP_014391467.1	Rha family transcriptional regulator	-
PmCQ6_RS00350	52920..53738	+	819	WP_015691057.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	35137..84693	49556

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-89)

Antitoxin

• Cro/C1-type helix-turn-helix domain (47-91)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11714.64 Da
• Isoelectric Point: 9.3582

>T113830 WP_016570073.1 NZ_CP033600:49459-49773 [Pasteurella multocida]
MLEIIETTIFNKWLKDLKDLSAKAAILARIGRAKNGNFGDHKSVGDGLYEMRIMKGAGYRVYYAQCKDVTYLLICGGDKS
TQKADIAKAKALWEEIKQKEELNV

Nucleotide

• Length: 315 bp

>T113830 NZ_CP033600:49459-49773 [Pasteurella multocida]
ATGCTTGAGATAATTGAAACAACAATATTTAATAAATGGCTTAAAGATCTAAAAGATTTGTCGGCTAAAGCGGCTATTCT
TGCTCGTATTGGTCGGGCTAAAAATGGTAATTTTGGCGATCATAAATCAGTTGGTGATGGTTTATATGAAATGCGAATAA
TGAAAGGAGCTGGTTATAGGGTTTATTATGCACAATGTAAAGATGTAACCTATTTGCTAATTTGCGGGGGTGATAAATCT
ACACAAAAAGCAGATATCGCTAAAGCAAAAGCATTATGGGAAGAGATTAAGCAAAAAGAGGAGCTAAATGTATGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10500.19 Da
• Isoelectric Point: 6.4688

>AT113830 WP_016570074.1 NZ_CP033600:49770-50060 [Pasteurella multocida]
MSKKVELKPFDIAEYLDSEEMIAAYLSEVLETGGTNEFISALGDVARARGMTELAAKTGLGRESLYKTLSHGSKPRFDTI
MKIVQALGIKLVPTHI

Nucleotide

• Length: 291 bp

>AT113830 NZ_CP033600:49770-50060 [Pasteurella multocida]
ATGAGTAAAAAAGTGGAATTAAAACCATTTGATATTGCTGAATACCTAGATAGTGAAGAAATGATTGCGGCATATTTAAG
TGAAGTTTTAGAAACAGGTGGCACTAATGAATTTATTTCTGCGCTAGGTGATGTCGCAAGAGCGAGAGGAATGACGGAGT
TAGCAGCAAAAACAGGATTAGGAAGAGAAAGTCTTTATAAAACATTATCACATGGAAGTAAGCCACGTTTTGATACTATA
ATGAAAATTGTGCAAGCATTAGGTATTAAACTTGTCCCAACGCATATCTAA

Structures

Toxin

• AlphaFold DB: V4NB29