Detailed information of TA system TA113823

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): spoIISABC/SpoIISA-SpoIISB
• Location: 3205463..3206380
• Replicon: chromosome
• Accession: NZ_CP033576
• Organism: Bacillus velezensis strain NY12-2

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: -
• Locus tag: EEB07_RS16600
• Protein ID: WP_123117762.1
• Coordinates: 3205634..3206380 (-)
• Length: 249 a.a.

Antitoxin (Protein)

• Gene name: spoIISB
• Uniprot ID: I2HQ14
• Locus tag: EEB07_RS16595
• Protein ID: WP_003154807.1
• Coordinates: 3205463..3205633 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EEB07_RS16560	3200696..3202318	+	1623	WP_123117761.1	pyocin knob domain-containing protein	-
EEB07_RS16565	3202331..3202702	+	372	WP_017417608.1	hypothetical protein	-
EEB07_RS16570	3202707..3202904	+	198	WP_007610833.1	XkdX family protein	-
EEB07_RS16575	3202961..3203722	+	762	Protein_3148	phage portal protein	-
EEB07_RS16580	3203774..3204037	+	264	WP_021493821.1	hemolysin XhlA family protein	-
EEB07_RS16585	3204051..3204314	+	264	WP_003154813.1	phage holin	-
EEB07_RS16590	3204328..3205206	+	879	WP_007407257.1	N-acetylmuramoyl-L-alanine amidase	-
EEB07_RS16595	3205463..3205633	-	171	WP_003154807.1	type II toxin-antitoxin system SpoIISB family antitoxin	Antitoxin
EEB07_RS16600	3205634..3206380	-	747	WP_123117762.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
EEB07_RS16605	3206484..3207482	-	999	WP_017417610.1	inorganic phosphate transporter	-
EEB07_RS16610	3207495..3208112	-	618	WP_003154804.1	DUF47 domain-containing protein	-
EEB07_RS16615	3208399..3209715	-	1317	WP_007610842.1	amino acid permease	-
EEB07_RS16620	3210037..3210987	+	951	WP_007610844.1	ring-cleaving dioxygenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (3-239)

Antitoxin

• Antitoxin SpoIISB (1-56)

Sequences

Toxin

Protein

• Length: 249 a.a.
• Molecular weight: 29049.56 Da
• Isoelectric Point: 4.8725

>T113823 WP_123117762.1 NZ_CP033576:c3206380-3205634 [Bacillus velezensis]
MLLFFQIMVWTMAAALILYVYASWRYEAKVKEKMFAIRKTWYLLFVLGSMVYWTYDPESLFAAWRQYLIVAVCFALIDAF
IFLSAYIKKLAGNELETDTREILEENNEMLHSYLEKLKTYQYLLKNEPIHVYYGSTEAYAEGISRLLAAYAEKMNVTASL
CGYSAQSDKDRLTEHMPDTADVQSRLNRKDVYYDQKGRLVLIPFTVQNRHYVIKLTSENLLTEFDYLLFTSLTSIYDLML
PIEEEGDG

Nucleotide

• Length: 747 bp

>T113823 NZ_CP033576:c3206380-3205634 [Bacillus velezensis]
ATGCTTTTGTTTTTTCAGATTATGGTGTGGACGATGGCGGCCGCACTCATTTTATATGTGTACGCATCCTGGCGGTATGA
GGCAAAGGTAAAAGAAAAAATGTTTGCCATCCGTAAAACGTGGTATTTACTATTTGTACTGGGCTCCATGGTCTATTGGA
CATATGATCCCGAATCGCTCTTTGCCGCATGGAGACAATATTTGATTGTTGCCGTCTGCTTTGCCCTTATTGATGCGTTT
ATCTTTTTGAGCGCGTATATCAAAAAGCTCGCCGGCAATGAATTAGAGACGGATACGCGGGAAATTTTAGAAGAAAATAA
CGAAATGCTTCACTCCTATCTTGAAAAACTGAAAACCTATCAATATCTGTTGAAAAATGAACCGATCCATGTATATTATG
GAAGTACAGAAGCTTATGCGGAAGGCATCAGCAGACTGCTGGCCGCTTATGCCGAAAAAATGAATGTCACGGCTTCCTTG
TGCGGCTATTCCGCACAATCGGATAAGGACCGCTTGACGGAGCATATGCCTGATACGGCAGACGTTCAGTCCCGTCTTAA
CCGAAAAGACGTCTACTATGATCAAAAAGGCAGGCTTGTGCTTATTCCGTTTACAGTCCAAAACCGCCATTACGTGATCA
AACTCACGTCGGAAAACCTGTTGACGGAATTCGATTATTTGCTTTTCACATCGCTAACGAGCATTTATGATTTAATGCTG
CCAATAGAAGAGGAAGGTGACGGTTAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6676.74 Da
• Isoelectric Point: 10.9273

>AT113823 WP_003154807.1 NZ_CP033576:c3205633-3205463 [Bacillus velezensis]
MEQAFQNGQPEQRAKPFKMFKKRSMTSIASYQVSPHTARIFKENERLIDKYKQKKA

Nucleotide

• Length: 171 bp

>AT113823 NZ_CP033576:c3205633-3205463 [Bacillus velezensis]
ATGGAGCAGGCATTTCAAAACGGGCAGCCGGAACAAAGAGCGAAACCGTTCAAAATGTTTAAAAAACGTTCAATGACAAG
TATCGCAAGTTACCAGGTCAGCCCTCATACCGCCAGAATTTTCAAAGAAAACGAGCGGCTGATTGACAAGTATAAACAAA
AAAAAGCTTGA

Structures

Antitoxin

• AlphaFold DB: I2HQ14