Detailed information of TA system TA113821

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2422150..2422787
• Replicon: chromosome
• Accession: NZ_CP033576
• Organism: Bacillus velezensis strain NY12-2

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G4NU33
• Locus tag: EEB07_RS12395
• Protein ID: WP_003156187.1
• Coordinates: 2422437..2422787 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: I2HMS5
• Locus tag: EEB07_RS12390
• Protein ID: WP_003156188.1
• Coordinates: 2422150..2422431 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EEB07_RS12370	2418515..2419114	-	600	WP_003156193.1	rhomboid family intramembrane serine protease	-
EEB07_RS12375	2419207..2419572	+	366	WP_007609580.1	holo-ACP synthase	-
EEB07_RS12380	2419737..2420744	+	1008	WP_007410230.1	outer membrane lipoprotein carrier protein LolA	-
EEB07_RS12385	2420861..2422030	+	1170	WP_123117613.1	alanine racemase	-
EEB07_RS12390	2422150..2422431	+	282	WP_003156188.1	type II toxin-antitoxin system antitoxin EndoAI	Antitoxin
EEB07_RS12395	2422437..2422787	+	351	WP_003156187.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
EEB07_RS12400	2422905..2423726	+	822	WP_003156182.1	STAS domain-containing protein	-
EEB07_RS12405	2423731..2424096	+	366	WP_007609584.1	RsbT antagonist protein RsbS	-
EEB07_RS12410	2424099..2424500	+	402	WP_017419223.1	anti-sigma regulatory factor	-
EEB07_RS12415	2424512..2425519	+	1008	WP_007609589.1	PP2C family protein-serine/threonine phosphatase	-
EEB07_RS12420	2425583..2425912	+	330	WP_003156176.1	anti-sigma factor antagonist RsbV	-
EEB07_RS12425	2425909..2426391	+	483	WP_003156173.1	anti-sigma B factor RsbW	-
EEB07_RS12430	2426357..2427145	+	789	WP_003156171.1	RNA polymerase sigma factor SigB	-
EEB07_RS12435	2427145..2427747	+	603	WP_007410234.1	SpoIIE family protein phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-45)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12977.98 Da
• Isoelectric Point: 4.8781

>T113821 WP_003156187.1 NZ_CP033576:2422437-2422787 [Bacillus velezensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Nucleotide

• Length: 351 bp

>T113821 NZ_CP033576:2422437-2422787 [Bacillus velezensis]
TTGATTGTAAAGCGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGCGTACGCCCGGT
TTTAGTGATCCAAAATGATATCGGAAATCGCTTTAGCCCGACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATTGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGATTAACGGATAAAATAACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCCT
GCAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10562.92 Da
• Isoelectric Point: 6.4706

>AT113821 WP_003156188.1 NZ_CP033576:2422150-2422431 [Bacillus velezensis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Nucleotide

• Length: 282 bp

>AT113821 NZ_CP033576:2422150-2422431 [Bacillus velezensis]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACTTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGGACAACTCGCCATAACC
GTGATTTGATGAGACGCGGGTATATGGAAATGGCGAAAATCAACTTGAATATCTCTTCTGAGGCTCACTATGCTGAGTGC
GAAGCAGAAACGACCGTTGAGCGCTTAGTCAGCGGAGGATAA

Structures

Toxin

• PDB: 4MDX
• PDB: 4ME7
• PDB: 1NE8

Antitoxin

• AlphaFold DB: I2HMS5