Detailed information of TA system TA113792

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 172445..173220
• Replicon: plasmid p2016AM-0877
• Accession: NZ_CP033511
• Organism: Shigella flexneri strain 2016AM-0877

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: Q7BEG9
• Locus tag: EEL47_RS25275
• Protein ID: WP_000405245.1
• Coordinates: 172445..172927 (-)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: Q7BEG8
• Locus tag: EEL47_RS25280
• Protein ID: WP_010921625.1
• Coordinates: 172918..173220 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EEL47_RS25260 (EEL47_25300)	168860..170551	+	1692	WP_134800253.1	T3SS effector E3 ubiquitin-protein ligase IpaH2.5	-
EEL47_RS25265 (EEL47_25305)	170603..171811	+	1209	Protein_211	IS3 family transposase	-
EEL47_RS25270 (EEL47_25310)	171854..172377	-	524	Protein_212	IS3-like element ISSfl11 family transposase	-
EEL47_RS25275 (EEL47_25315)	172445..172927	-	483	WP_000405245.1	GNAT family N-acetyltransferase	Toxin
EEL47_RS25280 (EEL47_25320)	172918..173220	-	303	WP_010921625.1	DUF1778 domain-containing protein	Antitoxin
EEL47_RS25290 (EEL47_25330)	173492..173764	+	273	WP_000019158.1	hypothetical protein	-
EEL47_RS25295 (EEL47_25335)	173745..174952	+	1208	WP_094085525.1	IS91 family transposase	-
EEL47_RS25920	175124..175252	+	129	Protein_217	IS5/IS1182 family transposase	-
EEL47_RS25310 (EEL47_25350)	175401..176855	-	1455	WP_000701108.1	type 3 secretion system effector OspC2	-
EEL47_RS25315 (EEL47_25355)	177259..177878	+	620	Protein_219	IS1 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	ipaJ / ipaA / ipaD / ipaC / ipaB / ipgC / ipgB1 / ipgA / icsB / ipgD / ipgE / ipgF / mxiG / mxiH / mxiI / mxiJ / mxiK / mxiN / mxiL / mxiM / mxiE / mxiD / mxiC / mxiA / spa15 / spa47 / spa13 / spa32 / spa33 / spa24 / spa9 / spa29 / spa40 / virA / icsA/virG / papC / ospI / ipaH9.8 / ospG / ipaH1.4 / ospE1 / nleE / icsP/sopA / ospB / ospC4 / ospC2 / ospD2 / ospF / ospD1 / ipgB2 / ospE2 / ipaH2.5 / ospC2 / ipaH4.5 / ipaH7.8 / ospD3/senA / ospC1 / ospC3	1..222142	222142
	-	inside	IScluster/Tn	-	ospE2 / ipaH2.5 / ospC2 / ipaH4.5 / ipaH7.8 / ospD3/senA / ospC1	159749..219716	59967

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (29-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (19-94)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17750.52 Da
• Isoelectric Point: 7.0382

>T113792 WP_000405245.1 NZ_CP033511:c172927-172445 [Shigella flexneri]
MEINVTAPALLTDEHILQPFDCGNEVLSNWLRGRAMKNQMLNASRTFVICLEDTLRIVGYYSLATGSVTHAELGRSLRHN
MPNPVPVVLLGRLAVDVCTQGHGFGKWLLSDAIHRVVNLADQVGIKAVMVHAIDDDARAFYERFGFVQSVVAPNTLFYKV

Nucleotide

• Length: 483 bp

>T113792 NZ_CP033511:c172927-172445 [Shigella flexneri]
ATGGAAATAAATGTCACCGCGCCAGCATTGTTGACGGATGAGCATATACTTCAGCCATTTGACTGTGGAAATGAGGTGCT
AAGTAACTGGTTACGTGGTCGGGCTATGAAAAACCAGATGCTCAATGCTTCTCGCACGTTCGTTATTTGCTTGGAGGATA
CTTTACGTATTGTGGGATACTACTCATTAGCTACTGGTTCCGTCACTCATGCCGAGCTTGGTCGCAGTTTGCGGCATAAC
ATGCCCAATCCTGTCCCTGTTGTTCTGTTAGGGCGTCTGGCTGTTGATGTTTGTACCCAGGGGCATGGTTTCGGTAAATG
GCTACTAAGTGATGCCATTCACCGGGTTGTCAATCTGGCTGATCAGGTTGGCATTAAGGCTGTTATGGTACATGCAATTG
ATGATGACGCCAGAGCATTTTATGAGCGTTTTGGTTTTGTTCAGTCAGTTGTGGCACCTAATACCTTGTTTTATAAAGTC
TGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11689.28 Da
• Isoelectric Point: 4.9167

>AT113792 WP_010921625.1 NZ_CP033511:c173220-172918 [Shigella flexneri]
MEVFMSTAASVRKTPREHQINIRATDEERAVIDYAASLVNKNRTDFIMELAYQEAKNIILDQRLFVLDNERYDSFITQLE
APVQNAEGRERLMAVKPEWK

Nucleotide

• Length: 303 bp

>AT113792 NZ_CP033511:c173220-172918 [Shigella flexneri]
ATGGAGGTTTTTATGTCTACAGCTGCAAGCGTCCGTAAAACACCGCGAGAACATCAGATTAACATCCGGGCAACTGATGA
GGAACGAGCCGTTATTGATTATGCTGCGAGCTTGGTCAATAAGAACCGTACCGATTTCATCATGGAACTGGCGTACCAGG
AAGCTAAAAACATTATCCTGGATCAACGTTTATTTGTGCTGGATAACGAACGTTATGATTCGTTTATTACGCAACTGGAA
GCTCCTGTGCAGAATGCAGAAGGGCGTGAACGTCTGATGGCGGTAAAGCCTGAATGGAAATAA

Structures

Toxin

• AlphaFold DB: Q7BEG9

Antitoxin

• AlphaFold DB: Q7BEG8