Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 4058515..4059310 Replicon chromosome
Accession NZ_CP033510
Organism Shigella flexneri strain 2016AM-0877

Toxin (Protein)


Gene name yeeV Uniprot ID Q93EZ7
Locus tag EEL47_RS20525 Protein ID WP_000854917.1
Coordinates 4058936..4059310 (+) Length 125 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID D2ACA1
Locus tag EEL47_RS20520 Protein ID WP_001280958.1
Coordinates 4058515..4058889 (+) Length 125 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EEL47_RS20475 (4053929) 4053929..4054345 + 417 WP_000275180.1 AraC family transcriptional regulator -
EEL47_RS20480 (4054404) 4054404..4055101 + 698 WP_095033700.1 IS1-like element IS1B family transposase -
EEL47_RS26435 (4055284) 4055284..4055547 + 264 WP_000466628.1 DNA polymerase III subunit gamma/tau -
EEL47_RS20490 (4055667) 4055667..4055840 + 174 WP_000088744.1 DUF905 family protein -
EEL47_RS20495 (4055940) 4055940..4056761 + 822 WP_001234584.1 DUF932 domain-containing protein -
EEL47_RS20505 (4057103) 4057103..4057576 + 474 WP_005011597.1 antirestriction protein -
EEL47_RS20510 (4057592) 4057592..4058068 + 477 WP_005011624.1 RadC family protein -
EEL47_RS20515 (4058131) 4058131..4058352 + 222 WP_001220314.1 DUF987 domain-containing protein -
EEL47_RS20520 (4058515) 4058515..4058889 + 375 WP_001280958.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
EEL47_RS20525 (4058936) 4058936..4059310 + 375 WP_000854917.1 TA system toxin CbtA family protein Toxin
EEL47_RS20530 (4059307) 4059307..4059798 + 492 WP_000976828.1 DUF5983 family protein -
EEL47_RS20535 (4059810) 4059810..4060007 + 198 WP_000839282.1 DUF957 domain-containing protein -
EEL47_RS20540 (4060092) 4060092..4060652 + 561 Protein_3906 DUF4942 domain-containing protein -
EEL47_RS20545 (4060724) 4060724..4061728 + 1005 WP_001179707.1 IS110-like element ISSfl8 family transposase -
EEL47_RS20550 (4062010) 4062010..4062291 + 282 Protein_3908 DUF4942 domain-containing protein -
EEL47_RS20560 (4062763) 4062763..4063701 + 939 Protein_3909 glyoxylate/hydroxypyruvate reductase GhrA -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 4060724..4061728 1004


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(1-112)

Antitoxin

(18-117)


Sequences


Toxin        


Download         Length: 125 a.a.        Molecular weight: 14145.22 Da        Isoelectric Point: 7.7761

>T113785 WP_000854917.1 NZ_CP033510:4058936-4059310 [Shigella flexneri]
MKTLSDTHVREVSRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFVDERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
STCPRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Download         Length: 375 bp

>T113785 NZ_CP033510:4058936-4059310 [Shigella flexneri]
ATGAAAACATTATCCGACACACATGTACGGGAGGTATCTCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAT
CCGACTGCTGGACCAGCATTATGGCCTCACACTGAATGACACACCGTTTGTCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCAGTTAATTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCACCTGTCCCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCTCGCCGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 13793.62 Da        Isoelectric Point: 6.4651

>AT113785 WP_001280958.1 NZ_CP033510:4058515-4058889 [Shigella flexneri]
VSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLYYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRYQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPAPATTS

Download         Length: 375 bp

>AT113785 NZ_CP033510:4058515-4058889 [Shigella flexneri]
GTGTCAGACAAACTCTCCGGGATAACTCATCCCGATGACAATCACGACCGCCCCTGGTGGGGGCTTCCCTGCACAGTGAG
GCCCTGTTTTGGCGCTCGTCTGGTGCAAGAGGGTAACCGCCTGTATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGCT
TCAGCGACGCAGATGCATACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGATATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCAGCACCCGCAACCACCTCATAA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB Q93EZ7


Antitoxin

Source ID Structure
AlphaFold DB A0A0K4PAV2

References