Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 1234741..1235395 Replicon chromosome
Accession NZ_CP033510
Organism Shigella flexneri strain 2016AM-0877

Toxin (Protein)


Gene name cptA Uniprot ID Q0T101
Locus tag EEL47_RS06390 Protein ID WP_000244767.1
Coordinates 1234741..1235148 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID D2A730
Locus tag EEL47_RS06395 Protein ID WP_000354052.1
Coordinates 1235129..1235395 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EEL47_RS06370 (1230698) 1230698..1232431 - 1734 WP_000813187.1 single-stranded-DNA-specific exonuclease RecJ -
EEL47_RS06375 (1232437) 1232437..1233147 - 711 WP_000715213.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
EEL47_RS06380 (1233172) 1233172..1234068 - 897 WP_000806987.1 site-specific tyrosine recombinase XerD -
EEL47_RS06385 (1234180) 1234180..1234701 + 522 WP_001055867.1 flavodoxin FldB -
EEL47_RS06390 (1234741) 1234741..1235148 - 408 WP_000244767.1 protein YgfX Toxin
EEL47_RS06395 (1235129) 1235129..1235395 - 267 WP_000354052.1 FAD assembly factor SdhE Antitoxin
EEL47_RS06400 (1235638) 1235638..1236618 + 981 WP_000886062.1 tRNA-modifying protein YgfZ -
EEL47_RS06405 (1236814) 1236814..1237473 - 660 WP_000250274.1 hemolysin III family protein -
EEL47_RS06410 (1237637) 1237637..1237947 - 311 Protein_1208 N(4)-acetylcytidine aminohydrolase -
EEL47_RS06415 (1237992) 1237992..1239425 + 1434 WP_005051722.1 6-phospho-beta-glucosidase BglA -
EEL47_RS06420 (1239482) 1239482..1240225 - 744 WP_000951957.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 15978.91 Da        Isoelectric Point: 10.9373

>T113777 WP_000244767.1 NZ_CP033510:c1235148-1234741 [Shigella flexneri]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRCINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T113777 NZ_CP033510:c1235148-1234741 [Shigella flexneri]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTATTTGATTGCG
TTCGCAGCCAGCGGTGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10519.05 Da        Isoelectric Point: 5.1599

>AT113777 WP_000354052.1 NZ_CP033510:c1235395-1235129 [Shigella flexneri]
MDINNKARIHWACRRGMRELDISIMSFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEIMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT113777 NZ_CP033510:c1235395-1235129 [Shigella flexneri]
ATGGACATTAACAACAAAGCCCGAATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGTCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATAATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TIU0

Antitoxin

Source ID Structure
AlphaFold DB D2A730

References