Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 440433..441055 Replicon chromosome
Accession NZ_CP033504
Organism Serratia sp. LS-1

Toxin (Protein)


Gene name Hha Uniprot ID A0A8B4G7F9
Locus tag EDY99_RS02155 Protein ID WP_004940313.1
Coordinates 440433..440636 (-) Length 68 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID A0A2X2G5J9
Locus tag EDY99_RS02160 Protein ID WP_004940312.1
Coordinates 440687..441055 (-) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EDY99_RS02130 436241..436705 + 465 WP_033651100.1 type II secretion system protein M -
EDY99_RS02135 436721..437536 + 816 WP_052133745.1 A24 family peptidase -
EDY99_RS02140 437533..437895 - 363 WP_047572433.1 type II secretion system pilot lipoprotein GspS -
EDY99_RS02145 438153..439580 + 1428 WP_046688371.1 N-acetylglucosamine-binding protein GbpA -
EDY99_RS02150 439633..440151 + 519 WP_033651103.1 cytochrome b/b6 domain-containing protein -
EDY99_RS02155 440433..440636 - 204 WP_004940313.1 hemolysin expression modulator Hha Toxin
EDY99_RS02160 440687..441055 - 369 WP_004940312.1 Hha toxicity modulator TomB Antitoxin
EDY99_RS02165 441215..441568 - 354 WP_123061421.1 hypothetical protein -
EDY99_RS02175 441975..442688 + 714 WP_123061422.1 ABC transporter ATP-binding protein -
EDY99_RS02180 442685..443542 + 858 WP_033651105.1 metal ABC transporter permease -
EDY99_RS02185 443568..444446 + 879 WP_123061423.1 zinc ABC transporter substrate-binding protein -
EDY99_RS02190 444552..444692 - 141 WP_004940304.1 type B 50S ribosomal protein L36 -
EDY99_RS02195 444705..444959 - 255 WP_123061424.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(8-64)

Antitoxin

Biofilm formation regulator YbaJ

(1-118)


Sequences

Toxin        


Download         Length: 68 a.a.        Molecular weight: 8107.37 Da        Isoelectric Point: 6.9756

>T113746 WP_004940313.1 NZ_CP033504:c440636-440433 [Serratia sp. LS-1]
MTKTDYLMRLRKCTTIDTLERVIEKNKYELSDDELELFYSAADHRLAELTMNKLYDKIPTSVWKYVR

Download         Length: 204 bp

>T113746 NZ_CP033504:c440636-440433 [Serratia sp. LS-1]
ATGACTAAAACCGACTATCTGATGCGTTTACGTAAATGCACCACGATTGATACTCTCGAACGTGTTATTGAAAAAAATAA
GTACGAGCTTTCCGATGATGAACTGGAGCTGTTTTACTCAGCCGCCGATCATCGTTTGGCGGAGCTGACCATGAACAAGC
TGTATGACAAAATCCCCACCTCCGTTTGGAAATATGTCAGATAA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 14261.95 Da        Isoelectric Point: 4.4314

>AT113746 WP_004940312.1 NZ_CP033504:c441055-440687 [Serratia sp. LS-1]
MDEYSPKRHDIAQLKFLCENLYDEGIATLGDSHHGWVNDPTSSVNLQLNELIEHIASFVMSYKIKYMDESDLSELVEEYL
DDTYTLFSSYGINDSDLRRWQKTKARLFRMFSGEDICTTMKT

Download         Length: 369 bp

>AT113746 NZ_CP033504:c441055-440687 [Serratia sp. LS-1]
ATGGATGAGTATTCGCCTAAGCGACACGACATTGCTCAACTCAAATTCTTGTGCGAAAATTTGTATGATGAAGGTATCGC
CACCCTGGGAGACAGCCACCACGGGTGGGTCAACGATCCGACATCCTCCGTCAACCTTCAGCTTAATGAGTTGATCGAAC
ATATCGCTTCGTTTGTGATGAGTTATAAAATTAAATACATGGACGAATCCGATTTGTCGGAGTTAGTCGAGGAATATCTC
GACGATACCTATACGCTGTTCAGCAGCTACGGTATCAATGATTCCGACCTGCGCCGCTGGCAAAAAACCAAGGCGAGATT
ATTCAGAATGTTCTCAGGAGAGGACATCTGTACGACAATGAAAACTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A8B4G7F9

Antitoxin

Source ID Structure
AlphaFold DB A0A2X2G5J9

References