Detailed information of TA system TA113745

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 6605..7242
• Replicon: plasmid p3442-IncR
• Accession: NZ_CP033469
• Organism: Enterobacter cloacae strain E3442

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: -
• Locus tag: EEI76_RS22860
• Protein ID: WP_123061016.1
• Coordinates: 6832..7242 (+)
• Length: 137 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: -
• Locus tag: EEI76_RS22855
• Protein ID: WP_123061017.1
• Coordinates: 6605..6835 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EEI76_RS22810	1664..1891	-	228	WP_032663682.1	hypothetical protein	-
EEI76_RS22815	1936..2562	-	627	WP_123061149.1	ParA family protein	-
EEI76_RS22820	2768..3407	+	640	Protein_3	DUF1173 family protein	-
EEI76_RS22825	3425..4207	-	783	WP_123061150.1	site-specific integrase	-
EEI76_RS22830	4204..4539	-	336	WP_063154408.1	hypothetical protein	-
EEI76_RS22835	4546..4944	-	399	WP_164472317.1	hypothetical protein	-
EEI76_RS22840	4992..5321	-	330	WP_123061151.1	hypothetical protein	-
EEI76_RS22845	5349..5660	-	312	WP_123061152.1	hypothetical protein	-
EEI76_RS22850	5706..5912	-	207	WP_123061202.1	hypothetical protein	-
EEI76_RS22855	6605..6835	+	231	WP_123061017.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
EEI76_RS22860	6832..7242	+	411	WP_123061016.1	PIN domain-containing protein	Toxin
EEI76_RS22865	7324..7503	-	180	Protein_12	IS110 family transposase	-
EEI76_RS22870	7569..8198	-	630	WP_123061015.1	helix-turn-helix domain-containing protein	-
EEI76_RS22875	8405..8899	-	495	WP_123061153.1	conjugal transfer protein	-
EEI76_RS22885	9173..9696	+	524	Protein_15	IS1 family transposase	-
EEI76_RS22890	9779..10060	-	282	WP_123061203.1	cytoplasmic protein	-
EEI76_RS22895	10068..10850	-	783	WP_123061154.1	DsbA family protein	-
EEI76_RS22900	10843..11298	-	456	Protein_18	fertility inhibition protein FinO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	sciN/tssJ / tssG / tssF / icmF/tssM / KPHS_23120 / tle1 / tli1 / tli1 / vgrG/tssI / clpV/tssH / hcp/tssD / dotU/tssL / vasE/tssK / vipB/tssC / vipA/tssB	1..71078	71078
	-	flank	IS/Tn	-	-	9173..9445	272

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-118)

Antitoxin

• SpoVT-AbrB domain (7-48)

Sequences

Toxin

Protein

• Length: 137 a.a.
• Molecular weight: 14665.00 Da
• Isoelectric Point: 6.4606

>T113745 WP_123061016.1 NZ_CP033469:6832-7242 [Enterobacter cloacae]
VNKTYMLDTNICSFIMREQPEAVLKRLEQAVLRGHRIVVSAITWSEISFAARAAGPVTQQLAEAFCARLDAILPWDRAAV
DATTEVKVALRLAGTPPGPNDTAIAGHAIAAGAVLVTRIGGEFERVPGLMLEDWVK

Nucleotide

• Length: 411 bp

>T113745 NZ_CP033469:6832-7242 [Enterobacter cloacae]
GTGAACAAGACCTATATGCTCGACACGAACATCTGCTCATTCATCATGCGCGAGCAGCCGGAAGCGGTGCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGTCACCGTATCGTGGTCTCGGCCATCACCTGGTCTGAGATTTCATTCGCCGCCCGGGCTG
CAGGGCCGGTCACGCAGCAGCTGGCTGAAGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCCGCGGTG
GATGCGACCACGGAGGTAAAGGTGGCGCTGCGCCTCGCCGGGACGCCGCCAGGCCCGAACGACACGGCGATAGCCGGGCA
CGCCATCGCCGCCGGCGCGGTGCTGGTGACGAGGATCGGGGGAGAGTTTGAACGGGTACCGGGGTTAATGCTTGAAGACT
GGGTGAAATAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8654.78 Da
• Isoelectric Point: 4.7571

>AT113745 WP_123061017.1 NZ_CP033469:6605-6835 [Enterobacter cloacae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELVIVREGDSIILRPVRPTWGSFAQFEKADPDFMAERGDVVSDEGRFNL

Nucleotide

• Length: 231 bp

>AT113745 NZ_CP033469:6605-6835 [Enterobacter cloacae]
ATGAGAACCGTATCCATTTTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGCGATCTGGATTTTGAGGGCGTCAG
CGAGCTGGTGATTGTCCGGGAAGGAGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AAAAAGCCGATCCGGACTTTATGGCGGAGCGCGGGGACGTTGTCAGCGACGAAGGGCGATTTAACCTGTGA