Detailed information of TA system TA113744

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-YafN
• Location: 16899..17434
• Replicon: plasmid p3442-IMI-2
• Accession: NZ_CP033468
• Organism: Enterobacter cloacae strain E3442

Toxin (Protein)

• Gene name: relE
• Uniprot ID: A0A221ZN37
• Locus tag: EEI76_RS22440
• Protein ID: WP_000222768.1
• Coordinates: 16899..17186 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: EEI76_RS22445
• Protein ID: WP_123061107.1
• Coordinates: 17183..17434 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EEI76_RS23415	13341..13643	+	303	WP_194074838.1	hypothetical protein	-
EEI76_RS22415	13769..14377	+	609	WP_123061102.1	DUF2913 family protein	-
EEI76_RS22420	14374..15327	-	954	WP_123061103.1	Rpn family recombination-promoting nuclease/putative transposase	-
EEI76_RS22430	15604..16221	-	618	WP_123061105.1	recombinase family protein	-
EEI76_RS22435	16646..16870	-	225	WP_151413638.1	hypothetical protein	-
EEI76_RS22440	16899..17186	-	288	WP_000222768.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
EEI76_RS22445	17183..17434	-	252	WP_123061107.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
EEI76_RS22450	17696..17989	-	294	WP_123061108.1	CopG family transcriptional regulator	-
EEI76_RS22455	17989..18627	-	639	WP_123061109.1	AAA family ATPase	-
EEI76_RS22460	18780..19925	-	1146	Protein_22	translesion error-prone DNA polymerase V subunit UmuC	-
EEI76_RS22465	19987..20994	+	1008	WP_072204972.1	site-specific integrase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	blaIMI-2	-	1..78374	78374

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-83)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11115.13 Da
• Isoelectric Point: 10.4701

>T113744 WP_000222768.1 NZ_CP033468:c17186-16899 [Enterobacter cloacae]
MTYTVKFRDDALKEWLKLDKVIQQQFAKKLKKCSENPHIPSAKLRGIKDCYKIKLRASGFRLVYQVIDDQLIIAVVAVGK
RERSDVYNLASERMR

Nucleotide

• Length: 288 bp

>T113744 NZ_CP033468:c17186-16899 [Enterobacter cloacae]
ATGACGTATACGGTCAAATTCAGGGATGATGCGCTGAAAGAGTGGCTGAAGCTGGATAAAGTCATTCAACAGCAATTTGC
GAAAAAGCTAAAGAAGTGCAGTGAAAATCCGCACATCCCTTCAGCAAAACTGCGCGGGATAAAGGACTGCTACAAAATCA
AACTTCGAGCCTCTGGCTTTCGTCTGGTCTATCAGGTGATTGACGATCAATTAATTATCGCTGTTGTAGCTGTAGGAAAA
CGTGAACGTAGTGACGTTTATAATCTTGCCAGTGAACGAATGAGATAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9227.41 Da
• Isoelectric Point: 4.0780

>AT113744 WP_123061107.1 NZ_CP033468:c17434-17183 [Enterobacter cloacae]
MPNIILSDTSASVSELKKNPMATVNSGDGYPVAILNRNQPAFYCVPAELYEKMLDALDDQELVKLVAERSNQQLHDVDLD
SYL

Nucleotide

• Length: 252 bp

>AT113744 NZ_CP033468:c17434-17183 [Enterobacter cloacae]
ATGCCCAACATCATTCTCAGTGACACAAGCGCCAGTGTCAGCGAACTTAAGAAAAACCCAATGGCAACTGTCAACTCCGG
TGATGGCTACCCGGTTGCTATTCTTAACCGCAACCAGCCCGCCTTTTACTGTGTTCCGGCAGAGCTATACGAGAAAATGC
TCGATGCCCTGGACGATCAGGAACTGGTTAAGCTTGTCGCCGAACGCAGCAACCAGCAGCTGCATGACGTGGATCTGGAT
AGTTACTTATGA

Structures

Toxin

• AlphaFold DB: A0A221ZN37