Detailed information of TA system TA113741

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 22975..23612
• Replicon: plasmid p3442-FRI-1
• Accession: NZ_CP033467
• Organism: Enterobacter cloacae strain E3442

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: -
• Locus tag: EEI76_RS21835
• Protein ID: WP_123061016.1
• Coordinates: 22975..23385 (-)
• Length: 137 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: -
• Locus tag: EEI76_RS21840
• Protein ID: WP_123061017.1
• Coordinates: 23382..23612 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EEI76_RS21815	21205..21585	+	381	WP_123061013.1	relaxosome protein TraM	-
EEI76_RS21820	21624..21812	+	189	WP_164472303.1	hypothetical protein	-
EEI76_RS21825	22019..22648	+	630	WP_123061015.1	helix-turn-helix domain-containing protein	-
EEI76_RS21830	22714..22893	+	180	Protein_19	IS110 family transposase	-
EEI76_RS21835	22975..23385	-	411	WP_123061016.1	PIN domain-containing protein	Toxin
EEI76_RS21840	23382..23612	-	231	WP_123061017.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
EEI76_RS21845	24239..24457	+	219	WP_123061018.1	type II toxin-antitoxin system antitoxin CcdA	-
EEI76_RS21850	24459..24764	+	306	WP_123061019.1	type II toxin-antitoxin system toxin CcdB	-
EEI76_RS21855	24852..25631	+	780	WP_123061020.1	site-specific integrase	-
EEI76_RS21860	25935..26555	+	621	WP_123061021.1	ParA family protein	-
EEI76_RS21865	26608..26838	+	231	WP_017694173.1	partitioning protein	-
EEI76_RS21870	26871..27071	-	201	WP_017694174.1	hypothetical protein	-
EEI76_RS21875	27572..28252	+	681	WP_123061023.1	DNA methylase	-
EEI76_RS21880	28249..28473	+	225	WP_123061024.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	blaFRI-5	-	1..93658	93658

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-118)

Antitoxin

• SpoVT-AbrB domain (7-48)

Sequences

Toxin

Protein

• Length: 137 a.a.
• Molecular weight: 14665.00 Da
• Isoelectric Point: 6.4606

>T113741 WP_123061016.1 NZ_CP033467:c23385-22975 [Enterobacter cloacae]
VNKTYMLDTNICSFIMREQPEAVLKRLEQAVLRGHRIVVSAITWSEISFAARAAGPVTQQLAEAFCARLDAILPWDRAAV
DATTEVKVALRLAGTPPGPNDTAIAGHAIAAGAVLVTRIGGEFERVPGLMLEDWVK

Nucleotide

• Length: 411 bp

>T113741 NZ_CP033467:c23385-22975 [Enterobacter cloacae]
GTGAACAAGACCTATATGCTCGACACGAACATCTGCTCATTCATCATGCGCGAGCAGCCGGAAGCGGTGCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGTCACCGTATCGTGGTCTCGGCCATCACCTGGTCTGAGATTTCATTCGCCGCCCGGGCTG
CAGGGCCGGTCACGCAGCAGCTGGCTGAAGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCCGCGGTG
GATGCGACCACGGAGGTAAAGGTGGCGCTGCGCCTCGCCGGGACGCCGCCAGGCCCGAACGACACGGCGATAGCCGGGCA
CGCCATCGCCGCCGGCGCGGTGCTGGTGACGAGGATCGGGGGAGAGTTTGAACGGGTACCGGGGTTAATGCTTGAAGACT
GGGTGAAATAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8654.78 Da
• Isoelectric Point: 4.7571

>AT113741 WP_123061017.1 NZ_CP033467:c23612-23382 [Enterobacter cloacae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELVIVREGDSIILRPVRPTWGSFAQFEKADPDFMAERGDVVSDEGRFNL

Nucleotide

• Length: 231 bp

>AT113741 NZ_CP033467:c23612-23382 [Enterobacter cloacae]
ATGAGAACCGTATCCATTTTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGCGATCTGGATTTTGAGGGCGTCAG
CGAGCTGGTGATTGTCCGGGAAGGAGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AAAAAGCCGATCCGGACTTTATGGCGGAGCGCGGGGACGTTGTCAGCGACGAAGGGCGATTTAACCTGTGA