Detailed information of TA system TA113740

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PP_4151-4152/excise(antitoxin)
• Location: 3331..4367
• Replicon: plasmid p3442-FRI-1
• Accession: NZ_CP033467
• Organism: Enterobacter cloacae strain E3442

Toxin (Protein)

• Gene name: PP_4152
• Uniprot ID: -
• Locus tag: EEI76_RS21760
• Protein ID: WP_123061006.1
• Coordinates: 3792..4367 (+)
• Length: 192 a.a.

Antitoxin (Protein)

• Gene name: PP_4151
• Uniprot ID: -
• Locus tag: EEI76_RS21755
• Protein ID: WP_123061005.1
• Coordinates: 3331..3792 (+)
• Length: 154 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EEI76_RS23410	1..876	+	876	WP_123061002.1	incFII family plasmid replication initiator RepA	-
EEI76_RS21740	1794..2027	+	234	WP_181013416.1	DUF2767 family protein	-
EEI76_RS21745	2051..2374	+	324	Protein_3	NIPSNAP family protein	-
EEI76_RS21750	2443..3024	+	582	WP_123061004.1	DUF2913 family protein	-
EEI76_RS21755	3331..3792	+	462	WP_123061005.1	helix-turn-helix domain-containing protein	Antitoxin
EEI76_RS21760	3792..4367	+	576	WP_123061006.1	PIN domain-containing protein	Toxin
EEI76_RS21765	4473..4613	+	141	Protein_7	transposase domain-containing protein	-
EEI76_RS21770	4951..5400	-	450	WP_181013418.1	lysogenic conversion protein	-
EEI76_RS21775	6227..7441	-	1215	WP_123061007.1	IS91 family transposase	-
EEI76_RS21780	7450..7869	-	420	WP_123061083.1	hypothetical protein	-
EEI76_RS21785	8094..8198	+	105	Protein_11	restriction endonuclease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	blaFRI-5	-	1..93658	93658

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (8-115)

Antitoxin

• Helix-turn-helix domain, group 17 (84-132)

Sequences

Toxin

Protein

• Length: 192 a.a.
• Molecular weight: 21458.78 Da
• Isoelectric Point: 4.7194

>T113740 WP_123061006.1 NZ_CP033467:3792-4367 [Enterobacter cloacae]
MNHTPYPVVLDACVLYPSFLRDLLIRLGLTGLYQPKWSASIENEWQRNLLANRTDLTEDQIKRTATLMNKAIPDALVTGF
EPLIDSIDLPDVDDRHVAAAAIRSNAEIIVTLNLKDFPAPALDAFGIEVLHPDDFVMDLFDLNRALVLSAVTTQRSSLRK
PPVSVDEYLEALLRQGMAQTVKELSKYRMLI

Nucleotide

• Length: 576 bp

>T113740 NZ_CP033467:3792-4367 [Enterobacter cloacae]
ATGAACCACACACCTTATCCTGTAGTTTTAGATGCTTGCGTACTGTACCCGTCATTTCTACGGGATTTACTGATTCGATT
GGGGCTGACCGGATTGTACCAACCAAAATGGTCAGCCAGTATCGAAAATGAGTGGCAACGTAACCTGTTAGCAAACAGAA
CGGATCTAACTGAAGATCAAATTAAACGCACTGCCACGCTAATGAATAAAGCCATTCCTGATGCGTTGGTCACCGGCTTT
GAGCCTCTGATTGATAGCATTGATTTACCTGATGTAGATGATCGGCATGTTGCCGCAGCTGCTATCCGTAGCAATGCTGA
AATCATTGTGACGTTGAATCTTAAAGATTTTCCGGCACCTGCTCTTGATGCCTTTGGTATCGAAGTGTTGCATCCTGACG
ATTTTGTTATGGATTTATTTGATTTAAATAGAGCACTTGTGCTTTCGGCCGTAACTACGCAGCGCAGTAGTTTACGGAAA
CCGCCCGTGTCGGTTGATGAATATTTAGAAGCGTTGCTAAGGCAAGGTATGGCGCAAACCGTAAAAGAATTAAGCAAATA
CCGCATGCTGATTTAG

Antitoxin

Protein

• Length: 154 a.a.
• Molecular weight: 16951.44 Da
• Isoelectric Point: 5.9921

>AT113740 WP_123061005.1 NZ_CP033467:3331-3792 [Enterobacter cloacae]
MTNSILDSLSLPAKGEIEAAIRGQRELAAYLSTKMETQKISIQDADNVTHQIELPTSSLTLLMSILGELALGNAVQVVPV
HAELTTQEAANILNVSRPHMVKLLEDGKLPFHKTGRHRRVLFADLMKYKDQRDSDSNKAMQELADLSQELGLY

Nucleotide

• Length: 462 bp

>AT113740 NZ_CP033467:3331-3792 [Enterobacter cloacae]
ATGACAAACTCAATTCTTGATAGCCTTAGCCTTCCGGCTAAAGGGGAGATTGAGGCAGCTATACGTGGACAAAGGGAGCT
TGCCGCCTACCTCTCCACAAAAATGGAAACACAGAAAATTTCGATTCAGGATGCAGATAACGTAACTCATCAAATCGAAC
TACCTACGTCTTCACTGACGTTGCTTATGTCGATTTTGGGTGAACTGGCACTTGGTAACGCGGTGCAAGTCGTTCCTGTG
CATGCTGAATTGACCACGCAGGAAGCCGCCAATATTCTGAATGTTTCACGTCCTCATATGGTGAAACTGCTGGAAGATGG
GAAGCTGCCTTTTCATAAAACAGGTCGCCATCGCCGCGTGCTTTTCGCCGATTTGATGAAGTACAAGGATCAGCGAGATA
GCGATAGCAATAAAGCTATGCAGGAGTTGGCAGATCTAAGCCAAGAGCTTGGACTTTACTAA