Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/HicA-HicB
Location 4448655..4449260 Replicon chromosome
Accession NZ_CP033466
Organism Enterobacter cloacae strain E3442

Toxin (Protein)


Gene name hicA Uniprot ID D2TUP9
Locus tag EEI76_RS21540 Protein ID WP_012906750.1
Coordinates 4449081..4449260 (-) Length 60 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A822X3T2
Locus tag EEI76_RS21535 Protein ID WP_032619507.1
Coordinates 4448655..4449059 (-) Length 135 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EEI76_RS21515 4444158..4444709 + 552 WP_032619504.1 phage tail protein I -
EEI76_RS21520 4444712..4446889 + 2178 WP_123060975.1 phage tail protein -
EEI76_RS21525 4446889..4447467 + 579 WP_032619505.1 tail fiber assembly protein -
EEI76_RS21530 4447544..4448614 + 1071 WP_123060976.1 glycosyltransferase family 9 protein -
EEI76_RS21535 4448655..4449059 - 405 WP_032619507.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
EEI76_RS21540 4449081..4449260 - 180 WP_012906750.1 type II toxin-antitoxin system HicA family toxin Toxin
EEI76_RS21550 4449771..4450088 + 318 WP_045345247.1 hypothetical protein -
EEI76_RS21555 4450098..4450769 - 672 WP_039269186.1 SOS response-associated peptidase -
EEI76_RS21560 4451028..4451948 - 921 WP_123061001.1 hypothetical protein -
EEI76_RS21570 4452566..4453048 - 483 WP_003863115.1 SsrA-binding protein SmpB -
EEI76_RS21575 4453205..4453642 + 438 WP_003863117.1 type II toxin-antitoxin system RatA family toxin -
EEI76_RS21580 4453632..4453919 + 288 WP_023314692.1 RnfH family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 4410990..4450769 39779


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(4-59)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(3-126)


Sequences

Toxin        


Download         Length: 60 a.a.        Molecular weight: 6644.85 Da        Isoelectric Point: 10.9132

>T113739 WP_012906750.1 NZ_CP033466:c4449260-4449081 [Enterobacter cloacae]
MDSRNAIAMIEADGWYLVRVKGSHHQFKHPTKKGLVTVKHPQKDIPLPTLKSIKKQAGL

Download         Length: 180 bp

>T113739 NZ_CP033466:c4449260-4449081 [Enterobacter cloacae]
ATGGATAGCAGGAATGCAATAGCAATGATAGAAGCCGATGGGTGGTATCTGGTGAGAGTTAAAGGCAGTCATCACCAGTT
CAAACACCCAACGAAAAAGGGGCTGGTAACGGTAAAGCATCCACAGAAAGACATACCGTTACCAACACTGAAAAGCATCA
AAAAACAGGCGGGGCTCTAA

Antitoxin


Download         Length: 135 a.a.        Molecular weight: 14676.53 Da        Isoelectric Point: 4.2136

>AT113739 WP_032619507.1 NZ_CP033466:c4449059-4448655 [Enterobacter cloacae]
MLYPVAIDKGDSSFGVRVPDIPGCFSGGEDYQDAIESVREAIEAHIELLVEDGESVPEATTVENWLSDPEYAGAVWALVD
VDITRLMGKAEKINVTLPSLLIRRIDQFVAAHPEYGSRSGFLSRVAADKVINQR

Download         Length: 405 bp

>AT113739 NZ_CP033466:c4449059-4448655 [Enterobacter cloacae]
ATGTTGTACCCTGTTGCTATTGATAAAGGCGATTCATCCTTCGGCGTTCGCGTACCTGATATTCCTGGCTGCTTCTCTGG
CGGTGAAGATTATCAGGACGCGATCGAAAGCGTACGTGAAGCGATCGAGGCACACATCGAATTACTGGTTGAAGATGGCG
AGAGCGTTCCCGAAGCAACGACCGTCGAAAACTGGCTATCTGATCCGGAGTACGCCGGTGCGGTATGGGCCCTAGTTGAC
GTGGATATAACACGCCTGATGGGAAAAGCGGAAAAAATCAATGTGACGCTACCCTCATTACTGATCCGACGTATTGATCA
GTTTGTGGCCGCGCATCCTGAATATGGAAGCCGTTCGGGCTTCCTTTCTCGCGTCGCTGCTGACAAGGTTATTAATCAAC
GATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7D6XDK4

Antitoxin

Source ID Structure
AlphaFold DB A0A822X3T2

References