Detailed information of TA system TA113735

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/COG3657-dnstrm_HI1420
• Location: 2957254..2957814
• Replicon: chromosome
• Accession: NZ_CP033466
• Organism: Enterobacter cloacae strain E3442

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: -
• Locus tag: EEI76_RS14230
• Protein ID: WP_063401841.1
• Coordinates: 2957254..2957520 (+)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: A0A837FG03
• Locus tag: EEI76_RS14235
• Protein ID: WP_022650216.1
• Coordinates: 2957527..2957814 (+)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EEI76_RS14210	2953480..2954829	+	1350	WP_045337031.1	lysine-sensitive aspartokinase 3	-
EEI76_RS14215	2954923..2955867	+	945	WP_022650218.1	ketopantoate/pantoate/pantothenate transporter PanS	-
EEI76_RS14220	2955918..2956183	+	266	Protein_2737	DUF3811 domain-containing protein	-
EEI76_RS14225	2956184..2957056	-	873	WP_003860347.1	23S rRNA pseudouridine(2604) synthase RluF	-
EEI76_RS14230	2957254..2957520	+	267	WP_063401841.1	hypothetical protein	Toxin
EEI76_RS14235	2957527..2957814	+	288	WP_022650216.1	putative addiction module antidote protein	Antitoxin
EEI76_RS14240	2957811..2959442	-	1632	WP_003860350.1	Na/Pi cotransporter family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (4-81)

Antitoxin

• Uncharacterized protein HI1420 (3-90)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10056.51 Da
• Isoelectric Point: 8.5172

>T113735 WP_063401841.1 NZ_CP033466:2957254-2957520 [Enterobacter cloacae]
MKEIVQTESFRRWEQNLKDRRANGLAGDIRPVGEGISELRIHFGPGYRVYFKDQGNCIIVLLCGGDKSSQARDILMAKML
SNVSQWQE

Nucleotide

• Length: 267 bp

>T113735 NZ_CP033466:2957254-2957520 [Enterobacter cloacae]
ATGAAGGAGATCGTTCAGACAGAATCCTTCCGACGCTGGGAGCAAAATTTAAAGGACAGGCGGGCAAATGGCTTAGCGGG
CGACATTAGACCCGTGGGGGAAGGTATCAGTGAACTGAGGATCCACTTTGGCCCGGGCTACAGAGTCTATTTTAAGGACC
AGGGCAATTGCATCATCGTGCTGTTATGTGGTGGTGACAAAAGCAGCCAGGCCAGAGACATACTTATGGCAAAAATGCTG
AGCAATGTATCCCAATGGCAGGAGTGA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10087.57 Da
• Isoelectric Point: 5.7807

>AT113735 WP_022650216.1 NZ_CP033466:2957527-2957814 [Enterobacter cloacae]
MHKLTPYDPANALVDDEEIAVFMADALETGDSAYIAKALGVIARAKGMSTISQQTGLSREQLYRSFSDKGNPTLKTTLAV
MKALGLGLTIKPSGD

Nucleotide

• Length: 288 bp

>AT113735 NZ_CP033466:2957527-2957814 [Enterobacter cloacae]
ATGCATAAATTAACACCCTACGATCCAGCCAACGCACTGGTGGATGACGAGGAAATCGCTGTGTTTATGGCTGATGCATT
AGAGACGGGTGACTCAGCGTACATTGCTAAAGCGCTGGGCGTCATCGCCCGAGCGAAAGGGATGTCGACCATTTCCCAGC
AAACGGGCCTGTCACGAGAACAACTGTATCGATCATTCAGTGATAAGGGGAACCCAACGCTCAAAACCACGCTGGCGGTC
ATGAAAGCATTGGGCCTTGGGTTAACAATCAAACCCTCTGGGGATTAA

Structures

Antitoxin

• AlphaFold DB: A0A837FG03