Detailed information of TA system TA113734

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 2355689..2356488
• Replicon: chromosome
• Accession: NZ_CP033466
• Organism: Enterobacter cloacae strain E3442

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: -
• Locus tag: EEI76_RS11305
• Protein ID: WP_123060711.1
• Coordinates: 2356111..2356488 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: EEI76_RS11300
• Protein ID: WP_123060710.1
• Coordinates: 2355689..2356054 (+)
• Length: 122 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EEI76_RS11250	2351096..2351503	+	408	WP_032233521.1	hypothetical protein	-
EEI76_RS11255	2351575..2351802	+	228	WP_123060703.1	hypothetical protein	-
EEI76_RS11265	2352105..2352941	+	837	WP_123060705.1	hypothetical protein	-
EEI76_RS11270	2353035..2353268	+	234	WP_123060706.1	DUF905 domain-containing protein	-
EEI76_RS11275	2353372..2354188	+	817	Protein_2170	DUF945 domain-containing protein	-
EEI76_RS11280	2354188..2354442	+	255	WP_123060707.1	hypothetical protein	-
EEI76_RS11285	2354530..2354940	+	411	Protein_2172	antirestriction protein	-
EEI76_RS11290	2354952..2355416	+	465	WP_123060708.1	DNA repair protein RadC	-
EEI76_RS11295	2355445..2355666	+	222	WP_123060709.1	DUF987 domain-containing protein	-
EEI76_RS11300	2355689..2356054	+	366	WP_123060710.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
EEI76_RS11305	2356111..2356488	+	378	WP_123060711.1	TA system toxin CbtA family protein	Toxin
EEI76_RS11310	2356485..2356937	+	453	WP_123060712.1	hypothetical protein	-
EEI76_RS11315	2357006..2357209	+	204	WP_123060713.1	DUF957 domain-containing protein	-
EEI76_RS11320	2357291..2358133	+	843	WP_123060714.1	DUF4942 domain-containing protein	-
EEI76_RS11325	2358381..2359001	+	621	WP_032608268.1	DUF2238 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	2340886..2359001	18115

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (15-114)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13754.84 Da
• Isoelectric Point: 9.9983

>T113734 WP_123060711.1 NZ_CP033466:2356111-2356488 [Enterobacter cloacae]
MQTLSAIPARKAPSHPTPVEIWQRLLTYLLERHYGLSLNDTQFGNDGVIQQHIDAGLSLADALNFLVEKSELVRIDRPGF
SIQHQSPFISAIDILRARKAIGLMQRTGYKAVTCAISGRSSGGLK

Nucleotide

• Length: 378 bp

>T113734 NZ_CP033466:2356111-2356488 [Enterobacter cloacae]
ATGCAAACCTTATCCGCCATTCCGGCTCGGAAGGCACCATCACACCCAACGCCCGTTGAAATCTGGCAGCGACTTCTTAC
GTATCTGCTGGAAAGGCATTACGGCCTTTCGCTTAACGACACACAGTTTGGCAACGACGGTGTCATTCAGCAGCATATTG
ACGCCGGGCTTTCACTGGCAGATGCACTCAATTTTCTCGTTGAAAAATCTGAACTGGTACGAATTGATCGCCCCGGTTTC
TCCATTCAGCATCAGTCTCCGTTCATCAGCGCTATTGATATACTCCGGGCCAGAAAGGCCATCGGCCTGATGCAACGTAC
CGGTTATAAAGCGGTGACTTGCGCCATCAGTGGACGCTCTTCCGGAGGGCTGAAATGA

Antitoxin

Protein

• Length: 122 a.a.
• Molecular weight: 13398.24 Da
• Isoelectric Point: 5.9501

>AT113734 WP_123060710.1 NZ_CP033466:2355689-2356054 [Enterobacter cloacae]
MPNTTPVANHDISEPWWGLKPTVTPCFGARLVQEGNHLHYLADRSTIEGTFSDADLRHLDQAFPVLMKQLELMLVSGELN
PRNQHCVTLYAKGLTLDADTLGSCGYLYIAIYPTSGNSIPR

Nucleotide

• Length: 366 bp

>AT113734 NZ_CP033466:2355689-2356054 [Enterobacter cloacae]
ATGCCGAACACAACACCTGTAGCAAACCATGATATTTCTGAGCCCTGGTGGGGACTTAAACCCACCGTCACGCCATGCTT
TGGCGCACGACTGGTACAGGAAGGCAACCACCTGCATTATCTGGCTGACCGCAGCACTATCGAAGGAACATTCAGCGACG
CGGATTTACGTCATCTCGACCAGGCCTTTCCGGTACTAATGAAGCAGCTTGAGTTAATGCTGGTTTCCGGTGAACTCAAT
CCCCGCAATCAGCATTGCGTCACGTTGTATGCAAAAGGTCTGACCCTCGACGCAGATACACTGGGCAGTTGTGGTTACCT
TTATATCGCGATTTATCCGACGTCAGGCAACAGCATCCCCCGCTAA