Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 1069017..1069756 Replicon chromosome
Accession NZ_CP033466
Organism Enterobacter cloacae strain E3442

Toxin (Protein)


Gene name tacT Uniprot ID A0A3L9PBP9
Locus tag EEI76_RS04950 Protein ID WP_003857133.1
Coordinates 1069271..1069756 (+) Length 162 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID A0A837FCR9
Locus tag EEI76_RS04945 Protein ID WP_003857131.1
Coordinates 1069017..1069283 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EEI76_RS04925 1064493..1065479 + 987 WP_003857123.1 DUF979 domain-containing protein -
EEI76_RS04930 1065489..1066484 + 996 WP_123060505.1 DUF2891 domain-containing protein -
EEI76_RS04935 1066506..1067894 - 1389 WP_063401776.1 MFS transporter -
EEI76_RS04940 1068024..1068953 + 930 WP_123060506.1 LysR family transcriptional regulator -
EEI76_RS04945 1069017..1069283 + 267 WP_003857131.1 DUF1778 domain-containing protein Antitoxin
EEI76_RS04950 1069271..1069756 + 486 WP_003857133.1 GNAT family N-acetyltransferase Toxin
EEI76_RS04955 1069934..1071226 - 1293 WP_123060507.1 glycoside hydrolase -
EEI76_RS04960 1071331..1072089 - 759 WP_003857136.1 DeoR/GlpR family DNA-binding transcription regulator -
EEI76_RS04965 1072176..1072760 + 585 WP_001190898.1 GDP-mannose pyrophosphatase nudK -
EEI76_RS04970 1072845..1073603 + 759 WP_123060508.1 trans-aconitate 2-methyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(58-151)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17540.23 Da        Isoelectric Point: 9.9658

>T113732 WP_003857133.1 NZ_CP033466:1069271-1069756 [Enterobacter cloacae]
VGRVTAPEPLSSVHQLAEFVSGEAVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTQATGNLRRNM
PDPIPVIILARLAVDVSLRGNGLGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKAFYIHHGFKASQTQERTLFLRLP
Q

Download         Length: 486 bp

>T113732 NZ_CP033466:1069271-1069756 [Enterobacter cloacae]
GTGGGACGTGTAACAGCACCAGAGCCCTTATCCAGCGTCCATCAACTGGCGGAGTTCGTCAGCGGAGAGGCCGTGCTTGA
CGAATGGCTAAAACAAAGAGGTCTGAAAAACCAGGCGCTCGGCGCAGCCCGAACCTTTGTCGTTTGTAAAACGGGCACGA
AGCAGGTCGCGGGATTTTACTCTCTGGCTACCGGCAGCGTTAACCATACGCAAGCGACAGGGAATCTGCGGCGTAACATG
CCGGATCCCATTCCCGTGATTATTCTTGCCCGTCTGGCAGTTGATGTCTCGTTACGCGGAAACGGGTTGGGAGCCGATTT
ACTCCACGATGCGGTTTTACGCTGTTATCGGGTAGCTGAGAATATCGGGGTGCGGGCGATCATGGTTCATGCACTCACTG
AGGAAGCCAAAGCGTTTTATATCCACCATGGTTTTAAAGCATCGCAGACTCAGGAACGGACACTGTTTCTCCGGCTTCCT
CAGTAG

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10090.50 Da        Isoelectric Point: 4.3837

>AT113732 WP_003857131.1 NZ_CP033466:1069017-1069283 [Enterobacter cloacae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACQAAENVILDRRVFNFNDEQYAEFIDMLDAPVADDPAIEKLL
ARKPQWDV

Download         Length: 267 bp

>AT113732 NZ_CP033466:1069017-1069283 [Enterobacter cloacae]
ATGAAATCAGATGTTCAACTCAATCTCAGAGCCAAAGAATCCCAGCGGGCGCTTATCGATGCCGCCGCAGAGATCCTCCA
TAAATCACGCACGGACTTCATTCTGGAGATGGCCTGTCAGGCAGCCGAGAATGTGATCCTCGATCGACGTGTATTCAATT
TTAACGATGAACAGTACGCAGAGTTCATTGACATGCTCGATGCCCCGGTCGCGGACGATCCGGCCATAGAAAAACTGCTG
GCAAGGAAACCACAGTGGGACGTGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3L9PBP9

Antitoxin

Source ID Structure
AlphaFold DB A0A837FCR9

References