Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA(toxin)
Location 356159..357942 Replicon chromosome
Accession NZ_CP033466
Organism Enterobacter cloacae strain E3442

Toxin (Protein)


Gene name hipA Uniprot ID -
Locus tag EEI76_RS01645 Protein ID WP_123060388.1
Coordinates 356593..357942 (-) Length 450 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID A0A837FFV9
Locus tag EEI76_RS01640 Protein ID WP_003859317.1
Coordinates 356159..356596 (-) Length 146 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EEI76_RS01620 351178..352200 + 1023 WP_003859324.1 HTH-type transcriptional regulator GalS -
EEI76_RS01625 352496..353494 + 999 WP_017383382.1 galactose/glucose ABC transporter substrate-binding protein MglB -
EEI76_RS01630 353574..355094 + 1521 WP_003859320.1 galactose/methyl galactoside ABC transporter ATP-binding protein MglA -
EEI76_RS01635 355110..356120 + 1011 WP_003859318.1 galactose/methyl galactoside ABC transporter permease MglC -
EEI76_RS01640 356159..356596 - 438 WP_003859317.1 helix-turn-helix domain-containing protein Antitoxin
EEI76_RS01645 356593..357942 - 1350 WP_123060388.1 type II toxin-antitoxin system HipA family toxin Toxin
EEI76_RS01650 358077..358787 - 711 WP_003859314.1 outer membrane permeability protein SanA -
EEI76_RS01655 358914..359798 - 885 WP_003859312.1 cytidine deaminase -
EEI76_RS01660 359927..360622 - 696 WP_003859310.1 CidB/LrgB family autolysis modulator -
EEI76_RS01665 360619..361014 - 396 WP_003859308.1 CidA/LrgA family protein -
EEI76_RS01670 361183..362118 + 936 WP_003859306.1 tRNA dihydrouridine(16) synthase DusC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA-like, C-terminal

(166-405)

Antitoxin

Cro/C1-type helix-turn-helix domain

(75-128)


Sequences

Toxin        


Download         Length: 450 a.a.        Molecular weight: 51091.80 Da        Isoelectric Point: 4.6948

>T113725 WP_123060388.1 NZ_CP033466:c357942-356593 [Enterobacter cloacae]
METLTVQAYLNETWTDIALIKYPGSEKNDWNTTQLDYLTEYAINFLDYDDFHAVSVNHPVSLFFDDHGQPGWLRFIDDII
PAGASRRYWINALDISELPVGQQNFLLLKFGTMSPVGNLRIKESVPEWNKFASTKTFTVADVIDRAADFLDYAQERGAAA
GGAAGAGGEAPKLLLRCSETDAIWIDTWQNEPDNRDRYYLVKYPRGNRTEVDCNILRAEFHYYHELNAMGFSTIPVETMR
LEEGSHYPSLWLPRFDIVRNEQDQLVRLGMESVYSLLQKAPGTLLDHETTLRALINKITSSHMVQQQGYHFDIPGFVTEW
VRRDLLNIIFGNSDNHGRNTAFVKAENQIMLSPVYDFAPMKADPIGIPRTLKWSLSCESGGDYNFNTIAQTLAEWIPPAT
LLDALHETAVQLIDLPERLAARGVPEQIMEMPAMGFRYIPDKLSRWGLL

Download         Length: 1350 bp

>T113725 NZ_CP033466:c357942-356593 [Enterobacter cloacae]
ATGGAGACGTTAACCGTACAGGCATATCTGAACGAAACATGGACGGATATCGCTCTCATCAAATATCCTGGAAGTGAAAA
AAATGACTGGAATACCACGCAGTTAGACTATCTTACTGAGTATGCGATTAATTTTCTCGACTATGATGATTTTCACGCAG
TCTCAGTCAATCACCCTGTATCACTCTTTTTTGATGACCATGGCCAACCGGGATGGCTGCGATTTATCGACGATATCATT
CCCGCCGGAGCAAGCCGCCGTTACTGGATTAATGCGCTCGACATCAGTGAGTTACCCGTTGGCCAGCAAAATTTCTTATT
GCTGAAATTTGGCACAATGTCCCCTGTGGGGAATTTGCGCATAAAGGAATCCGTCCCAGAATGGAATAAATTCGCCAGCA
CTAAAACGTTTACGGTTGCGGACGTCATCGATCGTGCGGCCGATTTTCTGGATTATGCCCAGGAAAGAGGCGCGGCGGCG
GGTGGTGCAGCCGGTGCTGGCGGTGAAGCGCCTAAACTTTTGCTGCGCTGTAGTGAAACTGATGCCATCTGGATCGATAC
CTGGCAAAACGAGCCGGATAACCGGGACCGCTATTATTTGGTTAAATATCCACGCGGTAACAGGACAGAAGTGGATTGTA
ATATTCTGCGAGCTGAATTCCATTATTATCATGAGCTTAACGCGATGGGTTTTTCCACGATCCCAGTGGAAACAATGCGC
CTTGAAGAAGGTAGCCATTACCCGTCTCTGTGGTTACCGCGTTTCGACATAGTGAGAAATGAGCAGGACCAACTGGTCAG
GCTGGGTATGGAATCGGTGTATTCGCTATTACAAAAGGCACCCGGCACCCTCCTTGATCATGAGACAACGTTGCGCGCGT
TAATTAATAAAATCACCTCCAGCCATATGGTCCAGCAGCAGGGTTATCATTTTGATATTCCGGGATTTGTAACAGAATGG
GTGCGTCGCGATCTGCTAAATATTATTTTTGGAAATAGTGACAACCATGGGCGAAATACCGCCTTTGTGAAGGCTGAAAA
TCAAATTATGCTCTCCCCTGTATATGACTTTGCGCCGATGAAAGCCGATCCTATAGGGATACCAAGAACCCTGAAGTGGT
CATTATCTTGCGAATCAGGGGGGGATTATAATTTCAACACTATCGCACAAACGCTGGCGGAGTGGATCCCTCCCGCAACA
TTACTTGATGCTCTGCATGAAACAGCCGTTCAGCTTATTGATCTCCCGGAAAGGCTGGCGGCGCGAGGCGTTCCCGAACA
GATAATGGAAATGCCCGCGATGGGTTTTCGCTACATCCCGGATAAACTTTCCCGTTGGGGTTTATTATGA

Antitoxin


Download         Length: 146 a.a.        Molecular weight: 16185.64 Da        Isoelectric Point: 10.5154

>AT113725 WP_003859317.1 NZ_CP033466:c356596-356159 [Enterobacter cloacae]
MKTPNEAESELLQTLAQVRSVNKKRHHDEAEEPDKPSVVKRQRVTQGLTRISTLDRQAVLHAAIRDILLGKITQGEALKR
LRVEVLGLKQDEYAKLVSVSRKTLSDVENNRGNYSADVINKIFKPFGLQTGLVPVSKSLIASLFS

Download         Length: 438 bp

>AT113725 NZ_CP033466:c356596-356159 [Enterobacter cloacae]
ATGAAAACACCTAATGAAGCTGAATCAGAGCTTTTACAGACATTAGCTCAAGTACGCAGCGTAAATAAAAAGCGACATCA
TGACGAGGCGGAAGAGCCGGATAAGCCATCGGTAGTGAAGAGGCAACGCGTTACACAAGGCTTAACGCGGATTAGTACTC
TCGATCGCCAGGCTGTACTGCATGCAGCCATACGGGACATTTTGCTGGGGAAAATCACACAGGGAGAGGCGCTGAAAAGG
CTCAGGGTCGAGGTGTTAGGGCTGAAGCAGGATGAATATGCAAAGCTGGTCAGCGTATCCCGGAAAACATTGTCGGATGT
AGAAAATAACAGAGGCAATTATTCCGCTGACGTCATAAATAAAATCTTTAAACCCTTTGGGCTTCAAACCGGACTAGTGC
CCGTTTCAAAATCGCTTATCGCTTCACTTTTCTCATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
AlphaFold DB A0A837FFV9

References