Detailed information of TA system TA113724

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 1465241..1465873
• Replicon: chromosome
• Accession: NZ_CP033464
• Organism: Brevibacillus laterosporus strain 1821L

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: A0A0F7EEZ8
• Locus tag: EEL30_RS08365
• Protein ID: WP_003333534.1
• Coordinates: 1465523..1465873 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: EEL30_RS08360
• Protein ID: WP_173645602.1
• Coordinates: 1465241..1465519 (+)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EEL30_RS08335	1461025..1461357	+	333	WP_113758875.1	metalloregulator ArsR/SmtB family transcription factor	-
EEL30_RS08340	1461574..1461807	+	234	WP_158326458.1	gamma-type small acid-soluble spore protein	-
EEL30_RS08345	1462155..1463186	+	1032	WP_113758873.1	DUF4367 domain-containing protein	-
EEL30_RS08350	1463426..1463869	+	444	WP_158326459.1	hypothetical protein	-
EEL30_RS08355	1463873..1465078	+	1206	WP_158328455.1	alanine racemase	-
EEL30_RS08360	1465241..1465519	+	279	WP_173645602.1	ribbon-helix-helix protein, CopG family	Antitoxin
EEL30_RS08365	1465523..1465873	+	351	WP_003333534.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
EEL30_RS08370	1466173..1466379	+	207	WP_113758871.1	ATP-binding protein	-
EEL30_RS08375	1466804..1467037	+	234	WP_113758870.1	ATP-binding protein	-
EEL30_RS08380	1467024..1467824	+	801	WP_158330141.1	RNA polymerase sigma factor SigB	-
EEL30_RS08385	1467871..1470030	+	2160	WP_158328456.1	RNA-binding transcriptional accessory protein	-
EEL30_RS08390	1470102..1470227	-	126	WP_003333532.1	cortex morphogenetic protein CmpA	-
EEL30_RS08395	1470338..1470673	-	336	WP_113758867.1	hydrolase/acyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-113)

Antitoxin

• Ribbon-helix-helix protein, CopG (8-45)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12932.83 Da
• Isoelectric Point: 4.8553

>T113724 WP_003333534.1 NZ_CP033464:1465523-1465873 [Brevibacillus laterosporus]
VNVKRGDVFFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKTYGFDRDSVILLEQI
RTIDKQRLTDKITHLDDEMMERVNESLQISLGLIDF

Nucleotide

• Length: 351 bp

>T113724 NZ_CP033464:1465523-1465873 [Brevibacillus laterosporus]
GTGAATGTTAAACGTGGCGATGTATTCTTCGCGGACCTTTCCCCTGTGGTTGGCTCCGAGCAAGGTGGAGTCAGACCGGT
ATTAGTTATTCAAAACGATATTGGAAATCGATTTAGTCCGACGGTTATCGTCGCTGCGATCACTGCTCAGATTCAAAAGG
CAAAGCTGCCTACACATGTGGAAATCGATGCAAAAACATATGGGTTTGATCGTGACTCTGTCATCTTATTAGAACAAATA
AGAACCATTGATAAACAAAGGTTGACCGATAAAATCACTCATTTAGATGATGAGATGATGGAAAGAGTGAATGAATCACT
TCAGATCAGCCTCGGATTAATAGATTTTTAA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10729.43 Da
• Isoelectric Point: 9.6543

>AT113724 WP_173645602.1 NZ_CP033464:1465241-1465519 [Brevibacillus laterosporus]
MSKSNTKRIMISLPQHLLQEVDGVVQKERSNRSELIRQAMSLYLKERKKRFIRETMQRGYLEMAKINLNMASEAFEAEEE
ADLTLDRLVSGV

Nucleotide

• Length: 279 bp

>AT113724 NZ_CP033464:1465241-1465519 [Brevibacillus laterosporus]
TTGTCTAAAAGCAATACAAAACGTATCATGATCAGTTTGCCGCAACATTTATTGCAAGAGGTAGACGGAGTCGTGCAAAA
AGAGAGATCAAATCGAAGTGAATTGATTCGCCAAGCGATGAGTCTCTACCTCAAGGAGCGGAAAAAACGCTTTATACGAG
AAACAATGCAACGTGGCTATTTGGAAATGGCTAAAATCAATCTCAATATGGCATCGGAGGCTTTTGAAGCAGAAGAGGAA
GCCGATCTTACCTTAGACCGCTTAGTTAGCGGGGTGTAG

Structures

Toxin

• AlphaFold DB: A0A0F7EEZ8