Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 2040829..2041343 Replicon chromosome
Accession NZ_CP033460
Organism Staphylococcus debuckii strain SDB 2975

Toxin (Protein)


Gene name mazF Uniprot ID A0A3G4R0E2
Locus tag CNQ82_RS09785 Protein ID WP_075140914.1
Coordinates 2040829..2041179 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID B9DMI8
Locus tag CNQ82_RS09790 Protein ID WP_015900820.1
Coordinates 2041176..2041343 (-) Length 56 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CNQ82_RS09765 2037887..2038657 - 771 WP_047133044.1 RNA polymerase sigma factor SigB -
CNQ82_RS09770 2038632..2039111 - 480 WP_095104352.1 anti-sigma B factor RsbW -
CNQ82_RS09775 2039113..2039439 - 327 WP_123145101.1 anti-sigma factor antagonist -
CNQ82_RS09780 2039515..2040516 - 1002 WP_123145102.1 PP2C family protein-serine/threonine phosphatase -
CNQ82_RS09785 2040829..2041179 - 351 WP_075140914.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
CNQ82_RS09790 2041176..2041343 - 168 WP_015900820.1 hypothetical protein Antitoxin
CNQ82_RS09795 2041424..2042578 - 1155 WP_123145103.1 alanine racemase -
CNQ82_RS09800 2042641..2042994 - 354 WP_123145104.1 holo-ACP synthase -
CNQ82_RS09805 2042996..2043481 - 486 WP_123145670.1 PH domain-containing protein -
CNQ82_RS09810 2043486..2044967 - 1482 WP_123145105.1 PH domain-containing protein -
CNQ82_RS09815 2044960..2045430 - 471 WP_123145106.1 PH domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-112)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 13075.18 Da        Isoelectric Point: 10.0708

>T113719 WP_075140914.1 NZ_CP033460:c2041179-2040829 [Staphylococcus debuckii]
MMRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKSKYKLDKDSVILLEQIR
TVDKKRLKEKLTYLSDEKMKEIDNAIQISLGLTHRN

Download         Length: 351 bp

>T113719 NZ_CP033460:c2041179-2040829 [Staphylococcus debuckii]
ATGATGAGGAGAGGCGATGTTTACTTAGCTGATTTATCACCAGTGCAAGGTTCTGAACAAGGGGGAGTTAGACCTGTTGT
AATTATACAAAATGACACTGGAAATAAGTATAGCCCGACTGTTATTGTAGCTGCAATAACAGGTCGGATTAATAAAGCGA
AAATTCCAACTCATGTTGAAATCGAGAAGAGTAAATATAAACTGGACAAAGATTCAGTTATTTTACTTGAACAAATTAGA
ACAGTCGACAAGAAACGTCTAAAAGAAAAACTGACATATTTATCAGACGAAAAGATGAAAGAAATTGATAATGCGATTCA
AATCAGCTTAGGCTTGACACATCGCAATTAA

Antitoxin


Download         Length: 56 a.a.        Molecular weight: 6211.75 Da        Isoelectric Point: 3.6836

>AT113719 WP_015900820.1 NZ_CP033460:c2041343-2041176 [Staphylococcus debuckii]
MSTFNQNRSYSLEQSLKEGYAQMADLNLSLATEAFSVECEACDCNESYLAFDKDE

Download         Length: 168 bp

>AT113719 NZ_CP033460:c2041343-2041176 [Staphylococcus debuckii]
ATGTCAACTTTTAATCAGAATAGAAGTTATAGTTTAGAACAGTCACTGAAAGAAGGCTACGCACAGATGGCTGACTTAAA
TCTCTCCCTCGCAACGGAGGCATTTTCAGTAGAATGTGAAGCCTGTGATTGCAACGAATCATACCTAGCTTTCGACAAGG
ATGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3G4R0E2

Antitoxin

Source ID Structure
AlphaFold DB A0A380F5Y4

References