Detailed information of TA system TA113699

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 126220..126920
• Replicon: chromosome
• Accession: NZ_CP033431
• Organism: Burkholderia gladioli strain Burkholderia gladioli Co14

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: -
• Locus tag: EDD84_RS20450
• Protein ID: WP_123037249.1
• Coordinates: 126492..126920 (+)
• Length: 143 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: A0A3G3LIT1
• Locus tag: EDD84_RS20445
• Protein ID: WP_052142853.1
• Coordinates: 126220..126495 (+)
• Length: 92 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EDD84_RS20430	122629..123720	-	1092	WP_123037247.1	ubiquinol oxidase subunit II	-
EDD84_RS20435	123897..125288	+	1392	WP_123037248.1	PLP-dependent aminotransferase family protein	-
EDD84_RS20440	125427..125969	-	543	WP_013689242.1	hypothetical protein	-
EDD84_RS20445	126220..126495	+	276	WP_052142853.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
EDD84_RS20450	126492..126920	+	429	WP_123037249.1	type II toxin-antitoxin system VapC family toxin	Toxin
EDD84_RS20455	126992..127888	-	897	WP_123037250.1	LysR family transcriptional regulator	-
EDD84_RS20460	128026..129498	+	1473	WP_123037251.1	M20 family metallopeptidase	-
EDD84_RS20465	129596..130897	+	1302	WP_123037252.1	MFS transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-130)

Antitoxin

• SpoVT-AbrB domain (7-50)

Sequences

Toxin

Protein

• Length: 143 a.a.
• Molecular weight: 15416.67 Da
• Isoelectric Point: 7.9048

>T113699 WP_123037249.1 NZ_CP033431:126492-126920 [Burkholderia gladioli]
VTHLYMLDTNICSYIIRKAPSSVLQRLEACVNAQHRIVISAITYAEMRDGTLGKKASPKLPGIISEFVSRLDGVLPWDAA
AVDTATRIRMDLNARGTPIGYNDASIAGHALSSAAILVTHNAREFERVAGLRIEDWVNSSPA

Nucleotide

• Length: 429 bp

>T113699 NZ_CP033431:126492-126920 [Burkholderia gladioli]
GTGACGCATCTCTACATGCTCGATACGAATATTTGTTCGTACATCATTCGAAAGGCCCCCTCCTCGGTTCTCCAGCGGCT
TGAAGCCTGCGTCAATGCCCAACATCGGATCGTTATCTCCGCCATTACCTATGCGGAAATGCGCGACGGCACGCTTGGCA
AAAAAGCGTCTCCCAAATTACCCGGGATTATTTCGGAATTCGTATCGAGGCTCGACGGCGTGCTGCCTTGGGATGCCGCA
GCCGTCGATACGGCGACGCGAATTCGCATGGACCTCAACGCCAGGGGCACGCCGATCGGATACAACGATGCGTCGATTGC
CGGCCATGCCCTCTCGTCCGCCGCCATTCTCGTGACTCATAACGCACGCGAATTCGAGCGCGTCGCCGGCTTGCGAATTG
AAGATTGGGTTAATTCCAGCCCCGCTTAA

Antitoxin

Protein

• Length: 92 a.a.
• Molecular weight: 10522.67 Da
• Isoelectric Point: 4.4662

>AT113699 WP_052142853.1 NZ_CP033431:126220-126495 [Burkholderia gladioli]
MRTVSIFRNSRNQAIRIPKDLEYQGITELEIRRDGDTLILRPVRPSWTSFAELDRGEISDFLTDDRRDVIASEGRFDLGD
DDLKPESGPDR

Nucleotide

• Length: 276 bp

>AT113699 NZ_CP033431:126220-126495 [Burkholderia gladioli]
ATGCGCACCGTATCCATTTTCAGAAACTCACGAAATCAAGCGATCCGCATCCCCAAAGACCTCGAATATCAAGGGATAAC
GGAACTTGAAATCAGGCGCGACGGCGATACGCTCATATTGCGTCCGGTCAGACCCTCCTGGACCTCGTTCGCCGAACTGG
ATCGTGGCGAGATATCCGACTTCCTTACCGACGATCGCCGTGATGTGATTGCATCCGAGGGGCGCTTCGATCTCGGCGAT
GACGATCTGAAGCCGGAATCGGGGCCCGACCGGTGA

Structures

Antitoxin

• AlphaFold DB: A0A3G3LIT1