Detailed information of TA system TA113698

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/upstrm_HI1419-dnstrm_HI1420
• Location: 47421..48005
• Replicon: chromosome
• Accession: NZ_CP033431
• Organism: Burkholderia gladioli strain Burkholderia gladioli Co14

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: EDD84_RS20100
• Protein ID: WP_123037224.1
• Coordinates: 47421..47717 (+)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A560UNM0
• Locus tag: EDD84_RS20105
• Protein ID: WP_036030606.1
• Coordinates: 47721..48005 (+)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EDD84_RS20080	43571..44632	-	1062	WP_123037221.1	selenide, water dikinase SelD	-
EDD84_RS20085	44852..45688	+	837	WP_123037222.1	DMT family transporter	-
EDD84_RS20090	45732..46202	-	471	WP_013689305.1	Lrp/AsnC family transcriptional regulator	-
EDD84_RS20095	46359..47297	+	939	WP_123037223.1	2-dehydropantoate 2-reductase	-
EDD84_RS20100	47421..47717	+	297	WP_123037224.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
EDD84_RS20105	47721..48005	+	285	WP_036030606.1	putative addiction module antidote protein	Antitoxin
EDD84_RS20110	48063..48389	-	327	WP_036054555.1	hypothetical protein	-
EDD84_RS20115	48538..49713	-	1176	WP_123037225.1	ABC transporter substrate-binding protein	-
EDD84_RS20120	49851..51488	-	1638	WP_123037226.1	PLP-dependent aminotransferase family protein	-
EDD84_RS20125	51624..52907	+	1284	WP_060000940.1	4-aminobutyrate--2-oxoglutarate transaminase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (4-95)

Antitoxin

• Uncharacterized protein HI1420 (11-89)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11349.19 Da
• Isoelectric Point: 10.3328

>T113698 WP_123037224.1 NZ_CP033431:47421-47717 [Burkholderia gladioli]
MVTLLRSSEFTNWLSSLRDQRDKARIAARLISAQLGNFGEYRVMGEGVREMKVDFGPGYRIYYVRRAEIVYVLLCGGDKS
TQKKDIKRALQMARELKE

Nucleotide

• Length: 297 bp

>T113698 NZ_CP033431:47421-47717 [Burkholderia gladioli]
ATGGTAACTTTGCTGCGCTCCAGTGAATTCACAAACTGGCTTTCCAGCCTGCGTGATCAGCGCGACAAAGCCCGAATCGC
CGCACGCCTGATCAGTGCCCAGCTAGGCAATTTTGGCGAGTACCGCGTGATGGGCGAAGGCGTCCGCGAAATGAAGGTCG
ACTTCGGGCCAGGTTATCGCATCTACTATGTCCGTCGCGCCGAGATCGTCTATGTCCTTCTCTGCGGTGGCGATAAATCT
ACACAGAAAAAAGACATCAAGCGCGCGCTCCAGATGGCGCGCGAACTGAAGGAGTAA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10072.47 Da
• Isoelectric Point: 5.0484

>AT113698 WP_036030606.1 NZ_CP033431:47721-48005 [Burkholderia gladioli]
MPDYAEFDAADYLDNDETVAEFLAVAAEDPDPAVFLAAVAAVARARGMTKVASEAGLGRVTLHKALTPGAHPRYETVQKI
LRSLGLRMTFTVAH

Nucleotide

• Length: 285 bp

>AT113698 NZ_CP033431:47721-48005 [Burkholderia gladioli]
ATGCCCGATTACGCCGAATTCGATGCGGCCGACTATCTCGACAATGACGAAACCGTGGCGGAATTTCTCGCGGTTGCGGC
CGAGGATCCCGATCCCGCAGTGTTCCTTGCTGCCGTGGCCGCTGTCGCACGCGCGCGCGGGATGACCAAGGTGGCGTCGG
AAGCCGGCCTTGGACGGGTCACCTTGCACAAGGCGCTGACACCTGGCGCTCACCCGCGATATGAGACCGTGCAGAAGATC
CTGCGCTCGCTCGGCCTTCGCATGACCTTCACCGTCGCCCACTGA

Structures

Antitoxin

• AlphaFold DB: A0A560UNM0