Detailed information of TA system TA113694

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 4290320..4290884
• Replicon: chromosome
• Accession: NZ_CP033428
• Organism: Mycobacterium avium subsp. paratuberculosis strain MAPK_CN7/15

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: X7VBD8
• Locus tag: EC391_RS20580
• Protein ID: WP_003875391.1
• Coordinates: 4290513..4290884 (+)
• Length: 124 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A7L5MGM2
• Locus tag: EC391_RS20575
• Protein ID: WP_003875390.1
• Coordinates: 4290320..4290526 (+)
• Length: 69 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EC391_RS20555	4285573..4286571	+	999	WP_003875386.1	4-hydroxy-3-methylbut-2-enyl diphosphate reductase	-
EC391_RS20560	4286595..4287803	-	1209	WP_010949696.1	hypothetical protein	-
EC391_RS20565	4287934..4289007	+	1074	WP_010949695.1	redox-regulated ATPase YchF	-
EC391_RS20570	4289207..4290151	+	945	WP_003875389.1	HNH endonuclease	-
EC391_RS20575	4290320..4290526	+	207	WP_003875390.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
EC391_RS20580	4290513..4290884	+	372	WP_003875391.1	type II toxin-antitoxin system VapC family toxin	Toxin
EC391_RS20585	4290984..4291424	+	441	WP_010949694.1	nitroreductase family deazaflavin-dependent oxidoreductase	-
EC391_RS20590	4291480..4291755	+	276	WP_003878531.1	hypothetical protein	-
EC391_RS20595	4291857..4292255	+	399	WP_003875394.1	VOC family protein	-
EC391_RS20600	4292322..4292705	+	384	WP_003875395.1	hypothetical protein	-
EC391_RS20605	4292775..4293128	+	354	WP_003875396.1	hypothetical protein	-
EC391_RS20610	4293285..4293809	+	525	WP_033713819.1	glycoside hydrolase	-
EC391_RS20615	4294220..4294544	+	325	Protein_4029	antibiotic biosynthesis monooxygenase	-
EC391_RS20620	4294549..4295232	-	684	WP_003875399.1	hypothetical protein	-
EC391_RS20625	4295350..4295547	+	198	WP_016706054.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-112)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-42)

Sequences

Toxin

Protein

• Length: 124 a.a.
• Molecular weight: 13770.14 Da
• Isoelectric Point: 6.6003

>T113694 WP_003875391.1 NZ_CP033428:4290513-4290884 [Mycobacterium avium subsp. paratuberculosis]
MILVDTSVWIDHLHVADKRLIEFLTNDAIGCHQMVIEELALGAIRRRADLLRLLSNLRAFPLLTHAEVLHLVECHRLWGR
GLSAIDVHLLGSVALVDGARLWTRDKNLKAAGWDVGIAIVDEL

Nucleotide

• Length: 372 bp

>T113694 NZ_CP033428:4290513-4290884 [Mycobacterium avium subsp. paratuberculosis]
ATGATTCTCGTTGACACGTCGGTATGGATCGATCATCTGCACGTCGCCGACAAGCGACTCATAGAGTTCCTGACCAACGA
CGCAATCGGTTGCCACCAAATGGTGATCGAGGAGTTGGCATTGGGTGCGATCAGGCGGCGCGCCGATCTATTGCGCCTGC
TGTCCAATCTTCGGGCGTTCCCGTTGTTGACGCATGCGGAGGTCCTTCATCTCGTCGAATGCCACCGCTTGTGGGGCCGC
GGACTGAGTGCGATCGACGTCCATCTTCTCGGGTCGGTTGCTCTGGTCGACGGTGCACGGCTATGGACCAGGGACAAGAA
TTTAAAAGCGGCGGGCTGGGACGTGGGTATCGCGATCGTCGACGAGCTCTGA

Antitoxin

Protein

• Length: 69 a.a.
• Molecular weight: 7231.22 Da
• Isoelectric Point: 11.0634

>AT113694 WP_003875390.1 NZ_CP033428:4290320-4290526 [Mycobacterium avium subsp. paratuberculosis]
MRTTVTIDDALLAEAAELTGVTESVALLRQGLQTLVRVESARRLAALGGTDRKASVGPRRRAPADDSR

Nucleotide

• Length: 207 bp

>AT113694 NZ_CP033428:4290320-4290526 [Mycobacterium avium subsp. paratuberculosis]
ATGAGGACGACGGTGACGATTGACGACGCCTTATTGGCAGAGGCGGCCGAACTGACCGGGGTGACAGAGAGCGTGGCGCT
CCTCCGTCAGGGACTGCAGACACTCGTTCGGGTAGAGAGCGCCCGTCGGCTGGCGGCGCTCGGCGGCACGGATCGGAAGG
CATCGGTCGGCCCTCGCCGGAGGGCACCCGCCGATGATTCTCGTTGA

Structures

Toxin

• AlphaFold DB: A0A0E2WB37

Antitoxin

• AlphaFold DB: A0A7L5MGM2