Detailed information of TA system TA113685

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 481326..481890
• Replicon: chromosome
• Accession: NZ_CP033427
• Organism: Mycobacterium avium subsp. paratuberculosis strain MAPK_CN9/15

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: X7VBD8
• Locus tag: EC390_RS02160
• Protein ID: WP_003875391.1
• Coordinates: 481326..481697 (-)
• Length: 124 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A7L5MGM2
• Locus tag: EC390_RS02165
• Protein ID: WP_003875390.1
• Coordinates: 481684..481890 (-)
• Length: 69 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EC390_RS02115	476663..476860	-	198	WP_016706054.1	hypothetical protein	-
EC390_RS02120	476978..477661	+	684	WP_003875399.1	hypothetical protein	-
EC390_RS02125	477666..477990	-	325	Protein_409	antibiotic biosynthesis monooxygenase	-
EC390_RS02130	478401..478925	-	525	WP_033713819.1	glycoside hydrolase	-
EC390_RS02135	479082..479435	-	354	WP_003875396.1	hypothetical protein	-
EC390_RS02140	479505..479888	-	384	WP_003875395.1	hypothetical protein	-
EC390_RS02145	479955..480353	-	399	WP_003875394.1	VOC family protein	-
EC390_RS02150	480455..480730	-	276	WP_003878531.1	hypothetical protein	-
EC390_RS02155	480786..481226	-	441	WP_010949694.1	nitroreductase family deazaflavin-dependent oxidoreductase	-
EC390_RS02160	481326..481697	-	372	WP_003875391.1	type II toxin-antitoxin system VapC family toxin	Toxin
EC390_RS02165	481684..481890	-	207	WP_003875390.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
EC390_RS02170	482059..483003	-	945	WP_003875389.1	HNH endonuclease	-
EC390_RS02175	483203..484276	-	1074	WP_010949695.1	redox-regulated ATPase YchF	-
EC390_RS02180	484407..485615	+	1209	WP_010949696.1	hypothetical protein	-
EC390_RS02185	485639..486637	-	999	WP_003875386.1	4-hydroxy-3-methylbut-2-enyl diphosphate reductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-112)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-42)

Sequences

Toxin

Protein

• Length: 124 a.a.
• Molecular weight: 13770.14 Da
• Isoelectric Point: 6.6003

>T113685 WP_003875391.1 NZ_CP033427:c481697-481326 [Mycobacterium avium subsp. paratuberculosis]
MILVDTSVWIDHLHVADKRLIEFLTNDAIGCHQMVIEELALGAIRRRADLLRLLSNLRAFPLLTHAEVLHLVECHRLWGR
GLSAIDVHLLGSVALVDGARLWTRDKNLKAAGWDVGIAIVDEL

Nucleotide

• Length: 372 bp

>T113685 NZ_CP033427:c481697-481326 [Mycobacterium avium subsp. paratuberculosis]
ATGATTCTCGTTGACACGTCGGTATGGATCGATCATCTGCACGTCGCCGACAAGCGACTCATAGAGTTCCTGACCAACGA
CGCAATCGGTTGCCACCAAATGGTGATCGAGGAGTTGGCATTGGGTGCGATCAGGCGGCGCGCCGATCTATTGCGCCTGC
TGTCCAATCTTCGGGCGTTCCCGTTGTTGACGCATGCGGAGGTCCTTCATCTCGTCGAATGCCACCGCTTGTGGGGCCGC
GGACTGAGTGCGATCGACGTCCATCTTCTCGGGTCGGTTGCTCTGGTCGACGGTGCACGGCTATGGACCAGGGACAAGAA
TTTAAAAGCGGCGGGCTGGGACGTGGGTATCGCGATCGTCGACGAGCTCTGA

Antitoxin

Protein

• Length: 69 a.a.
• Molecular weight: 7231.22 Da
• Isoelectric Point: 11.0634

>AT113685 WP_003875390.1 NZ_CP033427:c481890-481684 [Mycobacterium avium subsp. paratuberculosis]
MRTTVTIDDALLAEAAELTGVTESVALLRQGLQTLVRVESARRLAALGGTDRKASVGPRRRAPADDSR

Nucleotide

• Length: 207 bp

>AT113685 NZ_CP033427:c481890-481684 [Mycobacterium avium subsp. paratuberculosis]
ATGAGGACGACGGTGACGATTGACGACGCCTTATTGGCAGAGGCGGCCGAACTGACCGGGGTGACAGAGAGCGTGGCGCT
CCTCCGTCAGGGACTGCAGACACTCGTTCGGGTAGAGAGCGCCCGTCGGCTGGCGGCGCTCGGCGGCACGGATCGGAAGG
CATCGGTCGGCCCTCGCCGGAGGGCACCCGCCGATGATTCTCGTTGA

Structures

Toxin

• AlphaFold DB: A0A0E2WB37

Antitoxin

• AlphaFold DB: A0A7L5MGM2