Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 140678..141321 Replicon plasmid pKPC2_115069
Accession NZ_CP033404
Organism Klebsiella pneumoniae strain WCHKP115069

Toxin (Protein)


Gene name vagD Uniprot ID D8L2J9
Locus tag DVJ73_RS02410 Protein ID WP_001044770.1
Coordinates 140905..141321 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag DVJ73_RS02405 Protein ID WP_001261282.1
Coordinates 140678..140908 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DVJ73_RS02370 135901..136680 - 780 WP_013214009.1 site-specific integrase -
DVJ73_RS02375 136864..137868 - 1005 WP_011977814.1 hypothetical protein -
DVJ73_RS02380 137898..138101 - 204 WP_011977813.1 hypothetical protein -
DVJ73_RS02385 138147..138668 - 522 WP_013214008.1 hypothetical protein -
DVJ73_RS02390 138726..139118 - 393 WP_011977811.1 hypothetical protein -
DVJ73_RS02395 139155..140099 - 945 WP_011977810.1 hypothetical protein -
DVJ73_RS02400 140260..140721 - 462 WP_014343465.1 hypothetical protein -
DVJ73_RS02405 140678..140908 + 231 WP_001261282.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
DVJ73_RS02410 140905..141321 + 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin Toxin
DVJ73_RS02415 141395..142957 + 1563 WP_004206609.1 AAA family ATPase -
DVJ73_RS02420 142942..143964 + 1023 WP_000361404.1 helicase UvrD -
DVJ73_RS02425 144220..144917 + 698 Protein_183 IS1 family transposase -
DVJ73_RS02430 144946..145218 + 273 Protein_184 transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaKPC-2 / blaCTX-M-65 / blaTEM-1B / rmtB - 1..154986 154986


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15106.52 Da        Isoelectric Point: 7.1084

>T113665 WP_001044770.1 NZ_CP033404:140905-141321 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Download         Length: 417 bp

>T113665 NZ_CP033404:140905-141321 [Klebsiella pneumoniae]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT113665 WP_001261282.1 NZ_CP033404:140678-140908 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT113665 NZ_CP033404:140678-140908 [Klebsiella pneumoniae]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GYM2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZG3

References