Detailed information of TA system TA113660

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 185519..186162
• Replicon: plasmid p1_115069
• Accession: NZ_CP033402
• Organism: Klebsiella pneumoniae strain WCHKP115069

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: -
• Locus tag: DVJ73_RS01130
• Protein ID: WP_014386165.1
• Coordinates: 185746..186162 (+)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: -
• Locus tag: DVJ73_RS01125
• Protein ID: WP_032408893.1
• Coordinates: 185519..185749 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DVJ73_RS01095	180592..181587	-	996	WP_000611945.1	NAD(P)H-quinone oxidoreductase	-
DVJ73_RS01100	181594..182742	-	1149	WP_014386168.1	ABC transporter ATP-binding protein	-
DVJ73_RS01105	182913..184070	-	1158	WP_032408895.1	acetylornithine deacetylase	-
DVJ73_RS01110	184086..184760	-	675	WP_014386166.1	DUF1028 domain-containing protein	-
DVJ73_RS01115	184765..185199	-	435	WP_000103648.1	RidA family protein	-
DVJ73_RS01125	185519..185749	+	231	WP_032408893.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
DVJ73_RS01130	185746..186162	+	417	WP_014386165.1	PIN domain-containing protein	Toxin
DVJ73_RS01135	186485..187453	+	969	WP_074422984.1	IS5 family transposase	-
DVJ73_RS01140	187633..188007	-	375	WP_032408891.1	hypothetical protein	-
DVJ73_RS01145	188063..188389	-	327	WP_013023807.1	hypothetical protein	-
DVJ73_RS01150	188386..189114	-	729	WP_001568058.1	plasmid SOS inhibition protein A	-
DVJ73_RS01155	189111..189542	-	432	WP_001568057.1	conjugation system SOS inhibitor PsiB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	aph(3')-Ia / dfrA12 / aadA2 / qacE / sul1 / mph(A)	-	1..208288	208288
	-	flank	IS/Tn	-	-	186485..187453	968

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-130)

Antitoxin

• SpoVT-AbrB domain (7-55)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15127.44 Da
• Isoelectric Point: 7.8841

>T113660 WP_014386165.1 NZ_CP033402:185746-186162 [Klebsiella pneumoniae]
MTKTYMLDTNICSYIMREQPEAVLKRLEQAVLRRHRIVVSAITYAEMRFGAIGKRASPRHVQLVDAFCARLDAILAWDRA
AVDATTEVKATLAAAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVAGLTFEDWVQ

Nucleotide

• Length: 417 bp

>T113660 NZ_CP033402:185746-186162 [Klebsiella pneumoniae]
ATGACCAAAACCTACATGCTGGATACTAATATCTGCTCTTATATCATGCGCGAACAACCGGAAGCGGTGCTGAAACGCCT
AGAGCAAGCGGTGTTGCGCCGTCACCGGATAGTGGTGTCAGCCATCACCTATGCCGAAATGCGTTTTGGTGCCATCGGTA
AAAGGGCTTCGCCCCGTCATGTGCAGCTGGTGGACGCATTCTGTGCGCGCCTTGATGCCATACTGGCTTGGGATCGGGCC
GCAGTTGACGCTACAACAGAGGTTAAGGCCACGCTAGCAGCTGCCGGCACGCCAATCGGCCCAAACGACACAGCCATTGC
TGGACATGCCATTGCCGCTGGCGCCATCCTGGTGACGAATAATACGCGTGAATTTGAGCGAGTCGCTGGTCTGACGTTTG
AAGACTGGGTGCAATAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8854.89 Da
• Isoelectric Point: 4.5112

>AT113660 WP_032408893.1 NZ_CP033402:185519-185749 [Klebsiella pneumoniae]
MRTVSVFRNGNNRAIRLPRDLDFDGVNELEIIREGDTLILRPVKPSWSSFRQEEKADADFMAERENVVSDEGRFEL

Nucleotide

• Length: 231 bp

>AT113660 NZ_CP033402:185519-185749 [Klebsiella pneumoniae]
ATGCGAACTGTATCTGTATTCAGGAACGGTAATAACCGGGCCATCCGTCTGCCCCGCGATCTGGACTTTGACGGCGTGAA
CGAACTGGAAATCATCCGCGAGGGCGATACCCTCATCCTGCGGCCGGTAAAACCCAGTTGGAGTTCGTTCCGCCAGGAGG
AAAAAGCTGACGCAGATTTTATGGCGGAGCGTGAAAATGTTGTTAGTGACGAGGGACGTTTTGAGCTATGA