Detailed information of TA system TA113659

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 161623..162368
• Replicon: plasmid p1_115069
• Accession: NZ_CP033402
• Organism: Klebsiella pneumoniae strain WCHKP115069

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: A0A331KSM6
• Locus tag: DVJ73_RS00985
• Protein ID: WP_032408901.1
• Coordinates: 161877..162368 (+)
• Length: 164 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: -
• Locus tag: DVJ73_RS00980
• Protein ID: WP_014386183.1
• Coordinates: 161623..161889 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DVJ73_RS00920	157175..157588	-	414	WP_013023817.1	helix-turn-helix domain-containing protein	-
DVJ73_RS00925	157589..157867	-	279	WP_004152721.1	helix-turn-helix domain-containing protein	-
DVJ73_RS00930	157857..158177	-	321	WP_014386190.1	type II toxin-antitoxin system RelE/ParE family toxin	-
DVJ73_RS00935	158258..158482	-	225	WP_014386189.1	hypothetical protein	-
DVJ73_RS00940	158493..158705	-	213	WP_014386188.1	hypothetical protein	-
DVJ73_RS00945	158767..159093	-	327	WP_014386187.1	hypothetical protein	-
DVJ73_RS00960	159730..160080	-	351	WP_014386186.1	hypothetical protein	-
DVJ73_RS00965	160077..160349	-	273	WP_032408902.1	hypothetical protein	-
DVJ73_RS00970	160539..161024	+	486	WP_014386185.1	hypothetical protein	-
DVJ73_RS00975	161268..161426	-	159	WP_014386184.1	type I toxin-antitoxin system Hok family toxin	-
DVJ73_RS00980	161623..161889	+	267	WP_014386183.1	DUF1778 domain-containing protein	Antitoxin
DVJ73_RS00985	161877..162368	+	492	WP_032408901.1	GNAT family N-acetyltransferase	Toxin
DVJ73_RS00990	162809..163060	-	252	WP_032408900.1	aldo/keto reductase	-
DVJ73_RS00995	163257..164849	-	1593	Protein_180	IS66 family transposase	-
DVJ73_RS01000	164880..165230	-	351	WP_014386180.1	IS66 family insertion sequence element accessory protein TnpB	-
DVJ73_RS01005	165227..165667	-	441	WP_014386179.1	IS66 family insertion sequence hypothetical protein	-
DVJ73_RS01010	165929..166684	-	756	WP_032408898.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	aph(3')-Ia / dfrA12 / aadA2 / qacE / sul1 / mph(A)	-	1..208288	208288

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (89-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (2-87)

Sequences

Toxin

Protein

• Length: 164 a.a.
• Molecular weight: 17667.46 Da
• Isoelectric Point: 8.8757

>T113659 WP_032408901.1 NZ_CP033402:161877-162368 [Klebsiella pneumoniae]
VGKVTAPVPLGTSHILAEFHCGEPVLDEWIKHRGLKNQSLGAARTFVVCRGNASQVLAYYSLATGSVTHAIAPGSLRRNM
PDPVPVIILARLAVDTSYHGQGLGADLLHDAVLRCCRVAENIGVRAVMVHALSDSARQFYIHHGFIPSQTQDRTLFLRLP
LIT

Nucleotide

• Length: 492 bp

>T113659 NZ_CP033402:161877-162368 [Klebsiella pneumoniae]
GTGGGCAAAGTAACGGCTCCGGTCCCACTCGGTACGTCACACATCCTCGCTGAGTTTCACTGTGGTGAGCCTGTACTGGA
CGAATGGATAAAGCACCGGGGACTAAAAAATCAGTCCCTGGGGGCGGCCAGAACGTTTGTGGTGTGCCGGGGAAATGCTT
CACAGGTGCTTGCCTACTACTCTCTCGCGACCGGCAGCGTCACTCATGCCATTGCGCCGGGAAGCCTGCGAAGGAATATG
CCCGATCCTGTACCTGTCATCATCCTGGCCCGTCTTGCTGTCGATACGAGTTATCACGGCCAGGGACTGGGGGCTGATTT
ACTGCATGATGCCGTATTACGTTGCTGTCGGGTGGCTGAGAACATTGGCGTGCGGGCCGTTATGGTTCATGCACTCTCTG
ACAGTGCCAGACAGTTCTACATACATCACGGATTTATCCCTTCACAAACGCAGGATAGAACCCTTTTTTTACGGTTGCCT
CTCATTACTTAG

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10090.66 Da
• Isoelectric Point: 5.1169

>AT113659 WP_014386183.1 NZ_CP033402:161623-161889 [Klebsiella pneumoniae]
MKSDVQLNIRAKEAQRALIDTAAEILHKSRTDFILEMACQAAENVILDRRMFNLNDEQYAEFIDMLDAPVTANPAMEKLL
ARKPQWAK

Nucleotide

• Length: 267 bp

>AT113659 NZ_CP033402:161623-161889 [Klebsiella pneumoniae]
ATGAAATCTGATGTTCAGCTCAACATCCGGGCGAAAGAAGCCCAGCGGGCTCTGATTGATACTGCCGCTGAGATTCTCCA
CAAATCACGCACTGATTTTATACTGGAGATGGCCTGTCAGGCGGCGGAAAACGTCATTCTTGACCGCCGTATGTTCAATC
TCAATGATGAACAGTACGCCGAGTTTATCGACATGCTGGATGCGCCGGTCACCGCTAACCCGGCGATGGAGAAACTCCTG
GCGAGAAAACCGCAGTGGGCAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A331KSM6