Detailed information of TA system TA113647

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 1580689..1581533
• Replicon: chromosome
• Accession: NZ_CP033401
• Organism: Escherichia coli strain WCHEC020031

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: B1LJY4
• Locus tag: EDM51_RS08560
• Protein ID: WP_000854686.1
• Coordinates: 1580689..1581072 (-)
• Length: 128 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A8E0J1S3
• Locus tag: EDM51_RS08565
• Protein ID: WP_001285602.1
• Coordinates: 1581153..1581533 (-)
• Length: 127 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EDM51_RS08520	1575692..1576183	-	492	WP_001297187.1	DUF1097 domain-containing protein	-
EDM51_RS08525	1576285..1576839	-	555	WP_001001902.1	molecular chaperone YcdY	-
EDM51_RS08530	1576863..1577600	-	738	WP_000283664.1	zinc-binding phosphatase	-
EDM51_RS08535	1577655..1578593	-	939	WP_000351311.1	glyoxylate/hydroxypyruvate reductase GhrA	-
EDM51_RS08545	1579064..1579905	-	842	Protein_1478	DUF4942 domain-containing protein	-
EDM51_RS08550	1579990..1580187	-	198	WP_000839253.1	DUF957 domain-containing protein	-
EDM51_RS08555	1580204..1580692	-	489	WP_032180032.1	hypothetical protein	-
EDM51_RS08560	1580689..1581072	-	384	WP_000854686.1	TA system toxin CbtA family protein	Toxin
EDM51_RS08565	1581153..1581533	-	381	WP_001285602.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
EDM51_RS08570	1581544..1582227	-	684	WP_000086768.1	hypothetical protein	-
EDM51_RS08575	1582246..1582467	-	222	WP_000692298.1	DUF987 domain-containing protein	-
EDM51_RS08580	1582530..1583006	-	477	WP_001186726.1	RadC family protein	-
EDM51_RS08585	1583022..1583507	-	486	WP_000214307.1	antirestriction protein	-
EDM51_RS08590	1583599..1584420	-	822	WP_001761104.1	DUF945 domain-containing protein	-
EDM51_RS08600	1584521..1584729	-	209	Protein_1488	DUF905 family protein	-
EDM51_RS08605	1584830..1585285	-	456	WP_000581506.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-113)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 128 a.a.
• Molecular weight: 14272.28 Da
• Isoelectric Point: 6.8614

>T113647 WP_000854686.1 NZ_CP033401:c1581072-1580689 [Escherichia coli]
MKTLPDTHVRAASRCPSPVEIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYVLVRTDQPGF
SAGAPSSQLINSIDILRARRATGLMTRDNYRMVNNITLGKHPEEAKQ

Nucleotide

• Length: 384 bp

>T113647 NZ_CP033401:c1581072-1580689 [Escherichia coli]
ATGAAAACATTACCCGACACACACGTACGGGCGGCATCGCGCTGCCCGTCTCCGGTTGAAATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTATGGCCTTACGTTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGGATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGTGCTGGTACGAACTGACCAGCCGGGATTC
AGCGCAGGAGCCCCGTCGTCTCAGTTAATCAACAGCATTGATATTCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCG
GGACAATTACAGAATGGTAAATAACATTACCCTGGGCAAGCATCCGGAGGAGGCGAAACAATGA

Antitoxin

Protein

• Length: 127 a.a.
• Molecular weight: 13938.69 Da
• Isoelectric Point: 5.0823

>AT113647 WP_001285602.1 NZ_CP033401:c1581533-1581153 [Escherichia coli]
VSDTLPGTTLPDDNKDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSNADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCDADTLGSCGYVYLAVYPATETESNPPE

Nucleotide

• Length: 381 bp

>AT113647 NZ_CP033401:c1581533-1581153 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACGCTTCCCGACGACAACAAAGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCAGACCGCGCCGGTATCAGAGGCCGGT
TCAGCAATGCGGATGCATACCATCTGGACCAGGCTTTTCCACTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGGCATCAGCATACCGTCACGCTGTATGCGAAAGGGCTGACCTGCGATGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCTGCGACCGAAACGGAAAGTAACCCTCCGGAATAG