Detailed information of TA system TA113636

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 133743..134368
• Replicon: chromosome
• Accession: NZ_CP033401
• Organism: Escherichia coli strain WCHEC020031

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: EDM51_RS01585
• Protein ID: WP_000911330.1
• Coordinates: 133970..134368 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: EDM51_RS01580
• Protein ID: WP_000450524.1
• Coordinates: 133743..133970 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EDM51_RS01555	129546..130016	-	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-
EDM51_RS01560	130016..130588	-	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
EDM51_RS01565	130734..131612	+	879	WP_001295469.1	4-hydroxy-tetrahydrodipicolinate synthase	-
EDM51_RS01570	131629..132663	+	1035	WP_001297320.1	outer membrane protein assembly factor BamC	-
EDM51_RS01575	132876..133589	+	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
EDM51_RS01580	133743..133970	+	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
EDM51_RS01585	133970..134368	+	399	WP_000911330.1	tRNA(fMet)-specific endonuclease VapC	Toxin
EDM51_RS01590	134515..135378	+	864	WP_001267507.1	neutral zinc metallopeptidase	-
EDM51_RS01595	135393..137408	+	2016	WP_000829323.1	tRNA cytosine(34) acetyltransferase TmcA	-
EDM51_RS01600	137482..137823	+	342	Protein_119	esterase	-
EDM51_RS01605	137905..138651	-	747	WP_053906593.1	IS21-like element ISEc12 family helper ATPase IstB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T113636 WP_000911330.1 NZ_CP033401:133970-134368 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T113636 NZ_CP033401:133970-134368 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTTATTGAAGGTTTCGTATCTCGTTTGGTAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT113636 WP_000450524.1 NZ_CP033401:133743-133970 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT113636 NZ_CP033401:133743-133970 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAAGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTTTGA

Structures

Antitoxin

• AlphaFold DB: A0A829CM33