Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 70225..70750 Replicon plasmid pOXA1_020031
Accession NZ_CP033400
Organism Escherichia coli strain WCHEC020031

Toxin (Protein)


Gene name ccdB Uniprot ID V0SSI5
Locus tag EDM51_RS00880 Protein ID WP_001159868.1
Coordinates 70225..70530 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID A0A223LLB0
Locus tag EDM51_RS00885 Protein ID WP_023909027.1
Coordinates 70532..70750 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EDM51_RS00865 66135..67301 - 1167 WP_000772446.1 plasmid-partitioning protein SopA -
EDM51_RS00870 67889..68644 - 756 WP_000852146.1 replication initiation protein RepE -
EDM51_RS00875 69418..70224 - 807 WP_000016982.1 site-specific integrase -
EDM51_RS00880 70225..70530 - 306 WP_001159868.1 type II toxin-antitoxin system toxin CcdB Toxin
EDM51_RS00885 70532..70750 - 219 WP_023909027.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
EDM51_RS00890 71384..71581 + 198 WP_000215657.1 hypothetical protein -
EDM51_RS00895 71578..71862 - 285 WP_000642771.1 hypothetical protein -
EDM51_RS00900 71882..73015 - 1134 WP_000545983.1 DUF3800 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid tet(B) / sul1 / qacE / aadA5 / mph(A) / aac(3)-IId / blaTEM-1B / aph(6)-Id / aph(3'')-Ib / sul2 / blaOXA-1 / aac(6')-Ib-cr - 1..91652 91652


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11706.51 Da        Isoelectric Point: 6.4674

>T113634 WP_001159868.1 NZ_CP033400:c70530-70225 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T113634 NZ_CP033400:c70530-70225 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGATATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTCTCAGATAAAGTCTCCCGTGAACTTTACCCGGTGG
TGCATATCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8354.31 Da        Isoelectric Point: 4.7405

>AT113634 WP_023909027.1 NZ_CP033400:c70750-70532 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTIQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT113634 NZ_CP033400:c70750-70532 [Escherichia coli]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACAACCATACAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCTGACGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4VUB
PDB 2VUB
PDB 7EPI
PDB 7EPG
PDB 3VUB
PDB 1X75
PDB 1VUB
PDB 7EPJ
PDB 3HPW
PDB 3G7Z

Antitoxin

Source ID Structure
AlphaFold DB A0A223LLB0

References