Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 21361..22004 Replicon plasmid pKPC12_015625
Accession NZ_CP033395
Organism Klebsiella pneumoniae strain 015625

Toxin (Protein)


Gene name vagD Uniprot ID D8L2J9
Locus tag DCD52_RS01275 Protein ID WP_001044770.1
Coordinates 21588..22004 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag DCD52_RS01270 Protein ID WP_001261282.1
Coordinates 21361..21591 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DCD52_RS01235 16584..17363 - 780 WP_013214009.1 site-specific integrase -
DCD52_RS01240 17547..18551 - 1005 WP_011977814.1 hypothetical protein -
DCD52_RS01245 18581..18784 - 204 WP_011977813.1 hypothetical protein -
DCD52_RS01250 18830..19351 - 522 WP_013214008.1 hypothetical protein -
DCD52_RS01255 19409..19801 - 393 WP_011977811.1 hypothetical protein -
DCD52_RS01260 19838..20782 - 945 WP_011977810.1 hypothetical protein -
DCD52_RS01265 20943..21404 - 462 WP_014343465.1 hypothetical protein -
DCD52_RS01270 21361..21591 + 231 WP_001261282.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
DCD52_RS01275 21588..22004 + 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin Toxin
DCD52_RS01280 22078..23640 + 1563 WP_004206609.1 AAA family ATPase -
DCD52_RS01285 23625..24647 + 1023 WP_000361404.1 helicase UvrD -
DCD52_RS01290 24903..25600 + 698 Protein_31 IS1 family transposase -
DCD52_RS01295 25629..25901 + 273 Protein_32 transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid blaKPC-12 - 1..55373 55373


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15106.52 Da        Isoelectric Point: 7.1084

>T113611 WP_001044770.1 NZ_CP033395:21588-22004 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Download         Length: 417 bp

>T113611 NZ_CP033395:21588-22004 [Klebsiella pneumoniae]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT113611 WP_001261282.1 NZ_CP033395:21361-21591 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT113611 NZ_CP033395:21361-21591 [Klebsiella pneumoniae]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GYM2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZG3

References