Detailed information of TA system TA113598

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relE-PumB/upstrm_HI1419-dnstrm_HI1420
• Location: 4548316..4548897
• Replicon: chromosome
• Accession: NZ_CP033381
• Organism: Methylomonas sp. LW13

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: JL719_RS20595
• Protein ID: WP_033159121.1
• Coordinates: 4548604..4548897 (-)
• Length: 98 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: A0A2N0V3W3
• Locus tag: JL719_RS20590
• Protein ID: WP_033159122.1
• Coordinates: 4548316..4548600 (-)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JL719_RS20575	4543936..4546347	-	2412	WP_033159124.1	carbamoyltransferase HypF	-
JL719_RS20580	4546347..4547384	-	1038	WP_033159123.1	hydrogenase expression/formation protein HypE	-
JL719_RS20585	4547449..4547910	-	462	WP_033159173.1	hypothetical protein	-
JL719_RS20590	4548316..4548600	-	285	WP_033159122.1	putative addiction module antidote protein	Antitoxin
JL719_RS20595	4548604..4548897	-	294	WP_033159121.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
JL719_RS20600	4549107..4549361	+	255	WP_033159120.1	prevent-host-death protein	-
JL719_RS20605	4549361..4549636	+	276	WP_033159119.1	plasmid stabilization protein	-
JL719_RS20610	4549847..4550104	+	258	WP_033159118.1	hypothetical protein	-
JL719_RS20615	4550169..4551278	-	1110	WP_033159117.1	hydrogenase formation protein HypD	-
JL719_RS20620	4551275..4551502	-	228	WP_033159116.1	HypC/HybG/HupF family hydrogenase formation chaperone	-
JL719_RS20625	4551585..4551866	-	282	WP_033159115.1	type II toxin-antitoxin system RelE/ParE family toxin	-
JL719_RS20630	4551863..4552075	-	213	WP_033159114.1	addiction module protein	-
JL719_RS20635	4552132..4552956	-	825	WP_033159113.1	hydrogenase nickel incorporation protein HypB	-
JL719_RS20640	4552956..4553297	-	342	WP_020483415.1	hydrogenase maturation nickel metallochaperone HypA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (4-96)

Antitoxin

• Uncharacterized protein HI1420 (5-91)

Sequences

Toxin

Protein

• Length: 98 a.a.
• Molecular weight: 10872.60 Da
• Isoelectric Point: 9.9662

>T113598 WP_033159121.1 NZ_CP033381:c4548897-4548604 [Methylomonas sp. LW13]
MNYVVQQTESFAKWLASLKDLRSKTAIYRRIDRAQTGNLGDIKAVGEGVCEMRIDLGAGYRVYYTIRNGMMIILLAGGDK
STQQSDIQRAIKLAKEV

Nucleotide

• Length: 294 bp

>T113598 NZ_CP033381:c4548897-4548604 [Methylomonas sp. LW13]
ATGAATTATGTCGTGCAGCAAACGGAATCGTTTGCAAAATGGCTGGCGTCTTTAAAAGACCTGCGCTCCAAAACCGCGAT
TTACCGCCGCATTGACCGGGCGCAGACCGGTAATCTTGGCGACATCAAAGCGGTGGGCGAAGGCGTTTGCGAGATGCGCA
TCGATCTCGGTGCGGGTTACCGCGTTTATTACACAATACGTAACGGCATGATGATTATTTTGTTGGCCGGCGGCGATAAG
TCTACCCAACAGTCTGATATTCAACGTGCGATTAAACTGGCTAAAGAGGTTTGA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10247.91 Da
• Isoelectric Point: 4.6412

>AT113598 WP_033159122.1 NZ_CP033381:c4548600-4548316 [Methylomonas sp. LW13]
MSEQLLPFDMITLLDSDEAVSEYLNQVLEEGDSDELIRALGYIAKAKGMAMIAKESGLGRESLYKALAPGAKPRFDTVLK
VIRALGVKLHAEPV

Nucleotide

• Length: 285 bp

>AT113598 NZ_CP033381:c4548600-4548316 [Methylomonas sp. LW13]
ATGAGTGAACAACTACTGCCCTTCGACATGATCACGCTATTGGATAGCGACGAAGCAGTCAGCGAATACCTTAACCAAGT
GCTGGAAGAAGGCGATAGCGACGAACTGATAAGAGCCTTGGGCTATATCGCCAAAGCCAAAGGCATGGCCATGATCGCCA
AAGAATCAGGCCTGGGCCGCGAAAGTTTGTACAAAGCGTTGGCCCCCGGTGCAAAACCGCGCTTCGACACGGTGTTGAAG
GTTATTCGGGCATTGGGTGTTAAGTTACACGCAGAGCCGGTTTGA

Structures

Antitoxin

• AlphaFold DB: A0A2N0V3W3