Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1-StbC
Location 4284428..4285070 Replicon chromosome
Accession NZ_CP033381
Organism Methylomonas sp. LW13

Toxin (Protein)


Gene name vapC Uniprot ID A0A2N0V2E8
Locus tag JL719_RS19285 Protein ID WP_033158368.1
Coordinates 4284651..4285070 (+) Length 140 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A2N0V2K6
Locus tag JL719_RS19280 Protein ID WP_033158369.1
Coordinates 4284428..4284664 (+) Length 79 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JL719_RS19270 4280394..4282865 + 2472 WP_033158469.1 TonB-dependent receptor -
JL719_RS19275 4283119..4284360 + 1242 WP_051670304.1 amidohydrolase family protein -
JL719_RS19280 4284428..4284664 + 237 WP_033158369.1 DNA-binding protein Antitoxin
JL719_RS19285 4284651..4285070 + 420 WP_033158368.1 type II toxin-antitoxin system VapC family toxin Toxin
JL719_RS19290 4285067..4285984 - 918 WP_033158367.1 class I SAM-dependent methyltransferase -
JL719_RS19295 4286106..4286894 - 789 WP_051670302.1 4,5-DOPA dioxygenase extradiol -
JL719_RS19300 4286921..4287799 - 879 WP_033158366.1 pirin family protein -
JL719_RS19305 4287826..4288425 - 600 WP_033158365.1 NAD(P)H-dependent oxidoreductase -
JL719_RS19310 4288528..4289427 + 900 WP_033158364.1 LysR family transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(2-122)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 140 a.a.        Molecular weight: 15683.02 Da        Isoelectric Point: 7.4646

>T113597 WP_033158368.1 NZ_CP033381:4284651-4285070 [Methylomonas sp. LW13]
MYLIDTNVISEIRKGSKTNPGVNQFFAGVAQNDHKLYISVVTVGELRRGVDLIYHRGDFSQGKLLENWLNQLMQEYRHHV
LPIDNEIALVWGKMRVPNPQHPLDKLIAATALIYDLTVVTRNVGDFAGTGIRVLNPFAD

Download         Length: 420 bp

>T113597 NZ_CP033381:4284651-4285070 [Methylomonas sp. LW13]
ATGTATTTGATTGACACGAATGTGATTAGCGAAATCCGCAAAGGCAGCAAGACAAATCCGGGCGTTAACCAGTTCTTTGC
CGGCGTCGCCCAAAATGATCACAAGCTGTATATTTCTGTCGTCACCGTCGGTGAATTGCGGCGCGGCGTGGATCTGATTT
ATCACCGCGGCGATTTTAGCCAAGGCAAGTTACTCGAGAACTGGCTAAACCAGCTTATGCAGGAATATCGCCATCATGTC
TTGCCTATCGACAACGAGATTGCGCTGGTTTGGGGGAAAATGCGCGTACCAAATCCTCAACACCCCTTGGATAAATTGAT
CGCTGCTACGGCGTTAATTTACGATCTTACCGTTGTCACGCGGAATGTTGGTGACTTTGCCGGCACAGGAATTCGGGTCC
TCAATCCATTCGCGGATTAA

Antitoxin


Download         Length: 79 a.a.        Molecular weight: 8531.62 Da        Isoelectric Point: 5.0484

>AT113597 WP_033158369.1 NZ_CP033381:4284428-4284664 [Methylomonas sp. LW13]
MPSLVVRNLDESLVKALKARAAAHHRSTEAEHRAILAEVLTGPQRKSFTEVLAAMPNVGSDTDFLRVDTESDLADVFD

Download         Length: 237 bp

>AT113597 NZ_CP033381:4284428-4284664 [Methylomonas sp. LW13]
ATGCCCAGCCTTGTTGTTAGAAATCTTGATGAATCGCTGGTTAAGGCTTTGAAAGCCAGAGCTGCCGCCCACCATCGTTC
CACAGAAGCAGAACATCGCGCCATCCTGGCGGAGGTTTTGACCGGGCCGCAACGGAAATCTTTTACGGAAGTGCTGGCCG
CCATGCCCAATGTCGGTTCGGATACTGACTTTCTGCGTGTGGATACCGAGAGCGATTTGGCTGATGTATTTGATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2N0V2E8

Antitoxin

Source ID Structure
AlphaFold DB A0A2N0V2K6

References