Detailed information of TA system TA113596

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/CC2985(antitoxin)
• Location: 4157554..4158082
• Replicon: chromosome
• Accession: NZ_CP033381
• Organism: Methylomonas sp. LW13

Toxin (Protein)

• Gene name: parE
• Uniprot ID: -
• Locus tag: JL719_RS18680
• Protein ID: WP_033159545.1
• Coordinates: 4157795..4158082 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: A0A126T1H2
• Locus tag: JL719_RS18675
• Protein ID: WP_033159544.1
• Coordinates: 4157554..4157805 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JL719_RS18635	4153166..4153516	-	351	WP_033159537.1	IS66 family insertion sequence element accessory protein TnpB	-
JL719_RS18640	4153504..4153815	-	312	WP_033159538.1	hypothetical protein	-
JL719_RS18645	4153864..4154364	-	501	WP_033159539.1	hypothetical protein	-
JL719_RS18650	4154364..4154636	-	273	WP_033159540.1	hypothetical protein	-
JL719_RS18655	4155147..4155407	+	261	WP_033159541.1	hypothetical protein	-
JL719_RS18660	4155397..4155783	+	387	WP_033159542.1	type II toxin-antitoxin system VapC family toxin	-
JL719_RS18665	4156166..4156636	+	471	WP_051670558.1	hypothetical protein	-
JL719_RS18670	4156853..4157272	-	420	WP_051670560.1	DUF1993 domain-containing protein	-
JL719_RS18675	4157554..4157805	+	252	WP_033159544.1	type II toxin-antitoxin system ParD family antitoxin	Antitoxin
JL719_RS18680	4157795..4158082	+	288	WP_033159545.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
JL719_RS18685	4158457..4159731	-	1275	WP_033159546.1	three-Cys-motif partner protein TcmP	-
JL719_RS18690	4159758..4160507	-	750	WP_033159547.1	IS5 family transposase	-
JL719_RS18695	4160594..4161331	-	738	WP_033159559.1	phage Gp37/Gp68 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	4141265..4205308	64043
	-	flank	IS/Tn	-	-	4159758..4160507	749

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-90)

Antitoxin

• Antitoxin ParD (3-70)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11109.68 Da
• Isoelectric Point: 5.4731

>T113596 WP_033159545.1 NZ_CP033381:4157795-4158082 [Methylomonas sp. LW13]
MQIKKTVLADEDLIDIYLYGSQNFGQSQAESYFAELNQAFSFLADNPWVANERPEFVPPVRIHPHGKHLIVYTVEDDFLL
IVRVLHQRMDIKQHI

Nucleotide

• Length: 288 bp

>T113596 NZ_CP033381:4157795-4158082 [Methylomonas sp. LW13]
ATGCAAATTAAAAAAACTGTTCTTGCCGATGAAGACCTCATCGACATCTACTTGTATGGCAGTCAAAACTTTGGACAATC
TCAAGCAGAAAGTTACTTTGCTGAACTCAATCAAGCATTTTCATTCTTAGCTGACAATCCCTGGGTAGCAAACGAGCGTC
CGGAATTCGTACCACCTGTTCGCATTCATCCTCATGGCAAACATCTAATTGTTTACACTGTCGAAGACGACTTTCTATTG
ATTGTTCGCGTTTTACACCAACGTATGGATATTAAACAGCACATCTAG

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9396.49 Da
• Isoelectric Point: 4.9803

>AT113596 WP_033159544.1 NZ_CP033381:4157554-4157805 [Methylomonas sp. LW13]
MATMNISLPDPMRDWVQTQIQDGKYSSSSDYVRDLIRRDQETRQQQQVLQAAITEGLKSGISNRSMDDVLREAQARLAAL
NAN

Nucleotide

• Length: 252 bp

>AT113596 NZ_CP033381:4157554-4157805 [Methylomonas sp. LW13]
ATGGCAACTATGAATATTTCACTACCCGACCCTATGCGTGATTGGGTTCAAACTCAGATTCAAGACGGCAAATACTCTAG
CAGCAGTGATTATGTGCGAGACCTGATACGCCGAGACCAAGAGACACGCCAACAACAACAAGTCCTACAAGCCGCCATCA
CCGAAGGCTTAAAAAGCGGTATCAGCAACCGCTCAATGGATGATGTATTGAGAGAGGCCCAAGCGAGACTGGCTGCTCTG
AATGCAAATTAA

Structures

Antitoxin

• AlphaFold DB: A0A126T1H2