Detailed information of TA system TA113592

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/-
• Location: 3141539..3142078
• Replicon: chromosome
• Accession: NZ_CP033381
• Organism: Methylomonas sp. LW13

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: JL719_RS14250
• Protein ID: WP_201798995.1
• Coordinates: 3141782..3142078 (+)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: JL719_RS14245
• Protein ID: WP_201798994.1
• Coordinates: 3141539..3141697 (+)
• Length: 53 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JL719_RS14230	3137675..3138496	+	822	WP_033158050.1	PEP-CTERM domain protein	-
JL719_RS14235	3138822..3139343	+	522	WP_033158051.1	DUF1097 domain-containing protein	-
JL719_RS14240	3139775..3141322	+	1548	WP_033158052.1	hypothetical protein	-
JL719_RS14245	3141539..3141697	+	159	WP_201798994.1	hypothetical protein	Antitoxin
JL719_RS14250	3141782..3142078	+	297	WP_201798995.1	VapC toxin family PIN domain ribonuclease	Toxin
JL719_RS14255	3142212..3142742	+	531	WP_033158053.1	transposase	-
JL719_RS14260	3142903..3144408	-	1506	WP_033158054.1	hypothetical protein	-
JL719_RS14265	3145120..3146226	+	1107	WP_033158055.1	3-isopropylmalate dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (1-120)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (2-43)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 10862.70 Da
• Isoelectric Point: 7.8248

>T113592 WP_201798995.1 NZ_CP033381:3141782-3142078 [Methylomonas sp. LW13]
MIHPLIIAELAFGAPPAPRTQTLNNLRQLRYCNQAGLQEVEDFIEREALYGLGCGLIDLQLLASVLITPGAKLWTLDKRL
AKLAERFGVAYRISPTTN

Nucleotide

• Length: 297 bp

>T113592 NZ_CP033381:3141782-3142078 [Methylomonas sp. LW13]
TTGATTCACCCTCTGATCATTGCAGAATTGGCCTTTGGCGCGCCACCCGCGCCGAGAACTCAAACTCTGAACAACCTCCG
CCAATTAAGGTATTGCAATCAAGCTGGTTTGCAGGAAGTGGAAGACTTTATCGAACGTGAAGCGCTATATGGCTTGGGTT
GCGGACTAATCGATTTGCAGTTGCTAGCCTCGGTTTTGATTACGCCCGGCGCCAAGCTGTGGACGTTGGACAAGCGGTTG
GCGAAACTGGCTGAGCGGTTTGGTGTGGCTTATCGAATATCGCCGACTACGAATTGA

Antitoxin

Protein

• Length: 53 a.a.
• Molecular weight: 5739.55 Da
• Isoelectric Point: 4.8779

>AT113592 WP_201798994.1 NZ_CP033381:3141539-3141697 [Methylomonas sp. LW13]
MADPDMDKAEIFREAMKTFVWVQAAKRLAALGGSLPEMTDIPRERGQTNSSA

Nucleotide

• Length: 159 bp

>AT113592 NZ_CP033381:3141539-3141697 [Methylomonas sp. LW13]
ATGGCCGACCCGGACATGGATAAAGCCGAAATCTTCCGGGAAGCGATGAAAACCTTTGTATGGGTTCAGGCCGCCAAACG
CTTAGCTGCCTTGGGCGGGAGCCTACCGGAGATGACTGATATTCCCCGAGAACGCGGCCAAACCAACTCGTCCGCATGA