Detailed information of TA system TA113589

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/upstrm_HI1419-dnstrm_HI1420
• Location: 2301745..2302338
• Replicon: chromosome
• Accession: NZ_CP033381
• Organism: Methylomonas sp. LW13

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: A0A2N0V718
• Locus tag: JL719_RS10425
• Protein ID: WP_033156495.1
• Coordinates: 2301745..2302035 (+)
• Length: 97 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: JL719_RS10430
• Protein ID: WP_033156494.1
• Coordinates: 2302045..2302338 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JL719_RS10405	2297052..2299445	-	2394	WP_051669939.1	TonB-dependent receptor	-
JL719_RS10410	2299737..2300714	-	978	WP_051669937.1	FecR family protein	-
JL719_RS10415	2300773..2301285	-	513	WP_033156720.1	RNA polymerase sigma factor	-
JL719_RS10420	2301312..2301587	+	276	WP_033156496.1	hypothetical protein	-
JL719_RS10425	2301745..2302035	+	291	WP_033156495.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
JL719_RS10430	2302045..2302338	+	294	WP_033156494.1	putative addiction module antidote protein	Antitoxin
JL719_RS10435	2302554..2303969	-	1416	WP_051669935.1	sel1 repeat family protein	-
JL719_RS10440	2304058..2304825	-	768	WP_033156493.1	hypothetical protein	-
JL719_RS10445	2305578..2306683	-	1106	Protein_2058	IS5 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (1-96)

Antitoxin

• Cro/C1-type helix-turn-helix domain (40-92)

Sequences

Toxin

Protein

• Length: 97 a.a.
• Molecular weight: 10722.25 Da
• Isoelectric Point: 9.8609

>T113589 WP_033156495.1 NZ_CP033381:2301745-2302035 [Methylomonas sp. LW13]
MIDIKKTDVYAQWLDNLRDIRARARVLARVERLAAGNPGDVKSIGEGVSEMRIDYGPGYRVYFTRRGNEIVILLAGGDKS
TQTADIKTAQELARNL

Nucleotide

• Length: 291 bp

>T113589 NZ_CP033381:2301745-2302035 [Methylomonas sp. LW13]
ATGATCGATATAAAGAAAACCGATGTTTATGCCCAATGGCTGGACAATCTTCGCGACATTCGTGCTCGCGCACGAGTTTT
GGCCAGGGTCGAACGTCTGGCTGCCGGCAATCCGGGTGACGTGAAGTCCATCGGCGAAGGTGTATCTGAAATGCGCATCG
ACTATGGCCCCGGCTATCGAGTGTATTTCACGCGGCGCGGCAACGAAATCGTCATTTTATTGGCCGGTGGCGATAAAAGC
ACGCAGACTGCCGATATCAAAACCGCTCAAGAACTTGCTCGTAACCTGTAG

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10587.06 Da
• Isoelectric Point: 5.1258

>AT113589 WP_033156494.1 NZ_CP033381:2302045-2302338 [Methylomonas sp. LW13]
MTKIIISRYDVAEHLRTTEEMAAYLDACIEEADGDAAFIAKALGDIARARGMSQIAKETGLSRESLYRSLDGEHIPSFDT
ILKVTKALGIHLHADPA

Nucleotide

• Length: 294 bp

>AT113589 NZ_CP033381:2302045-2302338 [Methylomonas sp. LW13]
ATGACAAAAATTATTATCAGCCGCTACGATGTGGCGGAGCACTTGAGAACCACCGAAGAAATGGCGGCCTATCTGGATGC
CTGCATTGAAGAAGCCGACGGCGATGCGGCCTTCATAGCCAAAGCGCTTGGCGATATTGCCCGCGCGCGTGGCATGTCGC
AGATTGCCAAAGAGACCGGACTTTCCCGCGAAAGCCTGTATCGATCCCTGGACGGCGAGCATATCCCTAGTTTCGATACG
ATTCTCAAGGTAACAAAGGCACTAGGCATTCATCTGCATGCCGACCCGGCCTGA

Structures

Toxin

• AlphaFold DB: A0A2N0V718