Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 917488..918134 Replicon chromosome
Accession NZ_CP033381
Organism Methylomonas sp. LW13

Toxin (Protein)


Gene name vapC Uniprot ID A0A2N0VAS9
Locus tag JL719_RS03995 Protein ID WP_033156083.1
Coordinates 917488..917904 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name VapB1 Uniprot ID A0A2N0VAV2
Locus tag JL719_RS04000 Protein ID WP_033156084.1
Coordinates 917904..918134 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JL719_RS03965 913905..914192 + 288 WP_020484745.1 2Fe-2S iron-sulfur cluster binding domain-containing protein -
JL719_RS03970 914213..914563 + 351 WP_033156080.1 2Fe-2S iron-sulfur cluster binding domain-containing protein -
JL719_RS03975 914602..915207 + 606 WP_051669826.1 nitrogen fixation protein NifQ -
JL719_RS03980 915279..915839 - 561 WP_033156169.1 hypothetical protein -
JL719_RS03985 915844..916545 - 702 WP_051669828.1 HupE/UreJ family protein -
JL719_RS03990 916671..917372 - 702 WP_129871341.1 hypothetical protein -
JL719_RS03995 917488..917904 - 417 WP_033156083.1 type II toxin-antitoxin system VapC family toxin Toxin
JL719_RS04000 917904..918134 - 231 WP_033156084.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
JL719_RS04005 918248..918907 + 660 WP_033156085.1 metallophosphoesterase -
JL719_RS04010 918899..919279 - 381 Protein_794 type II toxin-antitoxin system VapC family toxin -
JL719_RS04015 919263..919520 - 258 WP_033156086.1 type II toxin-antitoxin system prevent-host-death family antitoxin -
JL719_RS04020 919659..920282 - 624 WP_033156087.1 inorganic pyrophosphatase -
JL719_RS04025 920417..921475 - 1059 WP_033156088.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-126)

Antitoxin

SpoVT-AbrB domain

(7-42)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15238.28 Da        Isoelectric Point: 6.4821

>T113588 WP_033156083.1 NZ_CP033381:c917904-917488 [Methylomonas sp. LW13]
MSAHYLLDTNICIYIAKYNPPAVRERFARHAADELAMSVITLGELRFGAEKSQARERALAGINELNGLIPVYGLPEAAGE
HYGQIRAALQKIGQPIGNNDLWLAAHARSEGWILVTNNEREFVRVPGLQVDNWVNSSG

Download         Length: 417 bp

>T113588 NZ_CP033381:c917904-917488 [Methylomonas sp. LW13]
ATGAGCGCTCACTATCTGCTCGATACCAATATCTGCATTTATATTGCCAAATACAATCCGCCCGCGGTGCGCGAGCGTTT
TGCTCGCCATGCAGCCGATGAATTGGCAATGTCTGTAATAACCTTGGGCGAGTTGCGTTTCGGCGCGGAGAAAAGTCAGG
CCAGGGAAAGGGCGTTAGCCGGCATTAACGAACTTAACGGTTTGATTCCGGTATATGGCTTGCCGGAAGCAGCTGGCGAG
CATTACGGACAGATACGTGCGGCGCTGCAAAAGATCGGGCAACCCATCGGCAATAACGATTTATGGTTGGCGGCTCACGC
CAGATCGGAAGGCTGGATTTTGGTCACCAACAACGAACGCGAATTTGTGCGAGTGCCGGGGTTGCAAGTAGACAACTGGG
TAAACTCATCTGGCTGA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8567.57 Da        Isoelectric Point: 4.4091

>AT113588 WP_033156084.1 NZ_CP033381:c918134-917904 [Methylomonas sp. LW13]
MTYARIFQSGNSQAVRLPKEFRFNTDRVEIFRRGDEIVLRETPANAAAIFDALAALPDDLMSDGREDAPPQDREAL

Download         Length: 231 bp

>AT113588 NZ_CP033381:c918134-917904 [Methylomonas sp. LW13]
ATGACCTATGCCCGTATTTTCCAATCCGGCAATAGCCAGGCAGTGCGGCTACCCAAGGAATTTCGCTTCAATACTGACCG
AGTGGAAATTTTCCGCCGGGGCGATGAAATCGTATTGCGGGAAACGCCCGCCAACGCCGCCGCGATATTCGACGCCTTAG
CAGCATTGCCCGACGACTTAATGAGCGACGGCCGCGAAGATGCGCCGCCCCAGGATCGTGAGGCGCTTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2N0VAS9

Antitoxin

Source ID Structure
AlphaFold DB A0A2N0VAV2

References