Detailed information of TA system TA113586

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/upstrm_HI1419-dnstrm_HI1420
• Location: 157280..157885
• Replicon: chromosome
• Accession: NZ_CP033381
• Organism: Methylomonas sp. LW13

Toxin (Protein)

• Gene name: relE
• Uniprot ID: A0A2N0V4W0
• Locus tag: JL719_RS00655
• Protein ID: WP_033157513.1
• Coordinates: 157589..157885 (-)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: JL719_RS00650
• Protein ID: WP_033157514.1
• Coordinates: 157280..157585 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JL719_RS00635	153534..154277	+	744	WP_033157517.1	methylenetetrahydrofolate reductase	-
JL719_RS00640	154539..155336	-	798	WP_033157516.1	indole-3-glycerol phosphate synthase TrpC	-
JL719_RS00645	155696..156790	-	1095	WP_033157515.1	hypothetical protein	-
JL719_RS00650	157280..157585	-	306	WP_033157514.1	putative addiction module antidote protein	Antitoxin
JL719_RS00655	157589..157885	-	297	WP_033157513.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
JL719_RS00660	157925..158941	-	1017	WP_033157512.1	anthranilate phosphoribosyltransferase	-
JL719_RS00665	159318..160163	+	846	WP_033157511.1	hypothetical protein	-
JL719_RS00670	160230..161420	-	1191	WP_129871459.1	TIGR03862 family flavoprotein	-
JL719_RS00675	161482..161739	-	258	WP_033157509.1	Txe/YoeB family addiction module toxin	-
JL719_RS00680	161736..161972	-	237	WP_033157508.1	hypothetical protein	-
JL719_RS00685	162167..162637	-	471	WP_174843443.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (2-94)

Antitoxin

• Uncharacterized protein HI1420 (13-100)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 10966.71 Da
• Isoelectric Point: 9.9486

>T113586 WP_033157513.1 NZ_CP033381:c157885-157589 [Methylomonas sp. LW13]
MYTIKQLPAFSEWLFGLKDGLTHRRLARRLEKAQLGNLGDVKAVGGSVFEMREHFGPGWRMYYVQRGEVLIVMLGGGDKS
SQTADIAKAQRLAALLED

Nucleotide

• Length: 297 bp

>T113586 NZ_CP033381:c157885-157589 [Methylomonas sp. LW13]
ATGTACACAATCAAACAACTCCCCGCATTCAGCGAATGGCTGTTTGGCTTGAAAGATGGATTGACACACCGCCGCTTGGC
GCGCCGACTGGAAAAGGCCCAACTAGGCAATCTCGGTGACGTCAAAGCGGTAGGCGGGAGTGTGTTTGAAATGCGTGAAC
ATTTCGGCCCCGGCTGGCGGATGTACTATGTGCAGCGAGGCGAGGTGTTGATTGTGATGCTGGGTGGCGGCGATAAATCA
AGCCAGACCGCCGATATTGCGAAAGCTCAACGCCTAGCCGCGTTGTTGGAGGACTGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 10767.41 Da
• Isoelectric Point: 4.6286

>AT113586 WP_033157514.1 NZ_CP033381:c157585-157280 [Methylomonas sp. LW13]
MNEKIKIADLPVFDITEHLDSDQAIAEYLTVVLEEDDPAALANALGSIARARGMTEIAAAAGISREALYKALRADAQPRF
ETIAKVCKALGVKLTVQTIAA

Nucleotide

• Length: 306 bp

>AT113586 NZ_CP033381:c157585-157280 [Methylomonas sp. LW13]
ATGAACGAAAAAATCAAAATTGCCGATTTGCCCGTGTTCGACATTACCGAACATCTGGATAGCGATCAAGCCATTGCCGA
ATACCTAACCGTTGTTCTGGAAGAAGATGATCCGGCGGCCTTGGCCAATGCACTCGGCAGTATTGCCAGGGCGCGAGGCA
TGACCGAAATTGCCGCTGCCGCAGGCATCAGCCGCGAAGCCTTGTATAAAGCTTTACGCGCCGACGCCCAACCGCGCTTC
GAGACCATTGCCAAAGTCTGCAAGGCACTTGGGGTTAAGCTGACGGTGCAGACAATAGCGGCATGA

Structures

Toxin

• AlphaFold DB: A0A2N0V4W0