Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-YafN
Location 120121..120656 Replicon plasmid pL73-2
Accession NZ_CP033379
Organism Escherichia coli strain L73

Toxin (Protein)


Gene name relE Uniprot ID A0A0K4CX11
Locus tag EB670_RS24450 Protein ID WP_000222771.1
Coordinates 120121..120408 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID T1PXV2
Locus tag EB670_RS24455 Protein ID WP_001139206.1
Coordinates 120405..120656 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EB670_RS24430 115315..115860 - 546 Protein_128 Tn3 family transposase -
EB670_RS24435 115905..116510 - 606 WP_104209005.1 recombinase family protein -
EB670_RS24440 116605..119502 + 2898 WP_001553819.1 Tn3-like element Tn5403 family transposase -
EB670_RS24445 119631..119831 + 201 WP_000896262.1 DUF839 domain-containing protein -
EB670_RS24450 120121..120408 - 288 WP_000222771.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
EB670_RS24455 120405..120656 - 252 WP_001139206.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
EB670_RS24465 121269..124127 + 2859 WP_122927426.1 helicase -
EB670_RS24470 124138..124835 - 698 Protein_135 IS1 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid qnrS1 / blaTEM-1B / aph(6)-Id / aph(3'')-Ib / sul2 / dfrA14 / floR / tet(A) mrkA / mrkB / mrkC / mrkD / mrkF / mrkJ 1..167170 167170
- inside IScluster/Tn tet(A) - 109261..129311 20050


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-83)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11116.17 Da        Isoelectric Point: 10.5833

>T113584 WP_000222771.1 NZ_CP033379:c120408-120121 [Escherichia coli]
MTYTVKFRDDALKEWLRLDKTIQQQFAKKLKKCSENPHIASAKLRGLKDCYKIKLRASGFRLVYQVIDDMLIIAVVAVGK
RERSNVYHLASERMK

Download         Length: 288 bp

>T113584 NZ_CP033379:c120408-120121 [Escherichia coli]
ATGACTTATACAGTAAAATTCCGGGATGATGCACTGAAAGAATGGCTCAGACTGGACAAGACCATTCAGCAGCAGTTCGC
AAAAAAACTGAAAAAATGCAGTGAAAATCCTCATATAGCTTCTGCAAAACTACGTGGATTAAAGGACTGCTATAAAATCA
AACTTCGCGCATCAGGTTTTCGTCTGGTTTATCAGGTTATTGATGATATGTTAATTATTGCGGTCGTCGCAGTAGGTAAA
CGTGAACGCAGCAACGTTTATCATCTCGCCAGTGAACGGATGAAATAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9171.39 Da        Isoelectric Point: 4.0927

>AT113584 WP_001139206.1 NZ_CP033379:c120656-120405 [Escherichia coli]
MPSIILSDTSASVSELKKNPMATVSAGEGYPVAILNRNQPAFYCVPAELYERMLDALDDQELVKLVTERSSQPLHDVDLD
SYL

Download         Length: 252 bp

>AT113584 NZ_CP033379:c120656-120405 [Escherichia coli]
ATGCCCAGTATCATTTTGTCTGACACCAGTGCAAGCGTCAGTGAGCTGAAAAAAAATCCGATGGCAACTGTCAGTGCCGG
AGAGGGGTATCCTGTTGCCATTCTTAACCGGAATCAGCCAGCATTCTACTGTGTCCCTGCTGAGCTCTATGAAAGAATGC
TTGATGCCTTGGATGACCAGGAGCTGGTAAAACTGGTTACTGAACGCAGCAGTCAGCCTCTGCATGATGTGGACCTGGAC
AGTTATTTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0K4CX11

Antitoxin

Source ID Structure
AlphaFold DB T1PXV2

References