Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-DnaT
Location 100725..101247 Replicon plasmid pL73-2
Accession NZ_CP033379
Organism Escherichia coli strain L73

Toxin (Protein)


Gene name relE Uniprot ID G3CAN5
Locus tag EB670_RS24340 Protein ID WP_000220560.1
Coordinates 100966..101247 (+) Length 94 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID G3CAG1
Locus tag EB670_RS24335 Protein ID WP_000121743.1
Coordinates 100725..100976 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EB670_RS24300 96098..96328 + 231 WP_000051064.1 partitioning protein -
EB670_RS24305 96344..96637 + 294 WP_015060510.1 hypothetical protein -
EB670_RS24310 96639..97607 + 969 WP_077783213.1 IS5 family transposase -
EB670_RS24315 97667..98089 - 423 WP_031942371.1 DNA distortion polypeptide 3 -
EB670_RS24320 98157..98603 - 447 WP_001074386.1 hypothetical protein -
EB670_RS24325 98643..99479 - 837 WP_001050931.1 replication initiation protein -
EB670_RS24335 100725..100976 + 252 WP_000121743.1 plasmid stabilization protein Antitoxin
EB670_RS24340 100966..101247 + 282 WP_000220560.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
EB670_RS24345 101393..101716 + 324 WP_001181903.1 hypothetical protein -
EB670_RS24350 101761..102006 + 246 WP_000356546.1 hypothetical protein -
EB670_RS24355 101996..102211 + 216 WP_001180116.1 hypothetical protein -
EB670_RS24365 102478..103175 - 698 Protein_115 IS1 family transposase -
EB670_RS24370 103457..104065 + 609 WP_047929051.1 type 3 fimbria major subunit MrkA -
EB670_RS24375 104161..104862 + 702 WP_047929050.1 type 3 fimbria chaperone MrkB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid qnrS1 / blaTEM-1B / aph(6)-Id / aph(3'')-Ib / sul2 / dfrA14 / floR / tet(A) mrkA / mrkB / mrkC / mrkD / mrkF / mrkJ 1..167170 167170
- inside IScluster/Tn qnrS1 / blaTEM-1B / aph(6)-Id / aph(3'')-Ib / sul2 / dfrA14 / floR / tet(A) - 51919..102981 51062


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 94 a.a.        Molecular weight: 11005.83 Da        Isoelectric Point: 10.5938

>T113583 WP_000220560.1 NZ_CP033379:100966-101247 [Escherichia coli]
MTYELAFDRRALKEWQKLGHTIREQFKKKLAERLENPRVPAARLHGHADRYKIKLRASGYRLVYQVIDEKVVLLVIAVGR
RESSEVYQIADLR

Download         Length: 282 bp

>T113583 NZ_CP033379:100966-101247 [Escherichia coli]
ATGACCTATGAACTGGCTTTTGACCGTAGAGCACTGAAGGAATGGCAGAAACTCGGCCACACCATCCGTGAACAATTCAA
AAAGAAACTGGCAGAACGGCTGGAAAATCCACGCGTACCCGCAGCCCGGTTACATGGTCATGCTGATCGCTATAAAATCA
AACTTCGTGCATCTGGCTACAGACTTGTATACCAAGTCATTGATGAGAAAGTCGTTTTACTCGTTATTGCCGTTGGAAGA
AGGGAAAGCAGCGAAGTCTATCAGATCGCAGATTTGCGCTAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9194.43 Da        Isoelectric Point: 4.1683

>AT113583 WP_000121743.1 NZ_CP033379:100725-100976 [Escherichia coli]
MSYQILTTTAASITDLKKNPMGTVAEGEGDAVAILNRNEPAFYCVPPKLYAYYRELAEDAELNAVADERMKNPEIVKVNL
DDL

Download         Length: 252 bp

>AT113583 NZ_CP033379:100725-100976 [Escherichia coli]
ATGAGCTATCAAATTCTGACAACCACAGCGGCCAGTATTACTGACCTGAAAAAAAATCCTATGGGAACCGTAGCTGAAGG
TGAAGGGGACGCTGTTGCGATCCTGAACCGAAACGAACCGGCGTTCTATTGCGTTCCACCAAAACTTTACGCCTACTATC
GGGAACTCGCTGAAGATGCTGAGTTAAACGCTGTTGCTGATGAGCGCATGAAAAACCCGGAAATTGTGAAGGTTAACCTG
GATGACCTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB G3CAN5

Antitoxin

Source ID Structure
AlphaFold DB A0A3S5I301

References